CCKA receptors mediate slow depolarizations in cultured mammalian sympathetic neurons

Steven R Knoper, Alan G. Meehan, Sergey Purnyn, Jay S. Coggan, Todd L. Anthony, David L. Kreulen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of cholecystokinin octapeptide (CCK-8) was examined in guinea-pig celiac ganglion (CG) neurons in primary culture using standard intracellular recording techniques. Sulfated CCK-8 (CCK-8S; 1 μM) evoked slow depolarizing responses in 94% of CG neurons tested. In contrast, membrane potential was not affected by nonsulfated CCK-8 (CCK-8NS; 1 μM), CCK tetrapeptide (CCK-4; 1 μM), or gastrin (1 μM). The selective CCKA receptor antagonist L 364,718 potently inhibited CCK-8S-induced slow depolarizations(IC50 2.9 pM). In contrast, the selective CCKB receptor antagonist L 365,260 was a weak inhibitor of CCK-8S-induced slow depolarizations (IC50 1.3 μM). The depolarizing responses to CCK-8S were associated with an average increase in cell input resistance of 61%. Single electrode voltage clamp experiments indicated that CCK-8S-induced depolarizations were associated with a slow inward shift in holding current. Thus, the present findings indicate that guinea-pig cultured CG neurons are endowed with excitatory CCKA receptors the activation of which elicits a decrease in membrane conductance, thereby resulting in slow depolarizations.

Original languageEnglish (US)
Pages (from-to)65-69
Number of pages5
JournalEuropean Journal of Pharmacology
Volume232
Issue number1
DOIs
StatePublished - Feb 23 1993

Fingerprint

Sincalide
Sympathetic Ganglia
Neurons
Inhibitory Concentration 50
Guinea Pigs
Devazepide
Tetragastrin
Gastrins
Membrane Potentials
Electrodes
8-sulfocholecystokinin octapeptide
Membranes

Keywords

  • CCK receptors
  • Cholecystokinin
  • Electrophysiology
  • Primary cell culture
  • Slow depolarization
  • Sympathetic neurons (mammalian)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

CCKA receptors mediate slow depolarizations in cultured mammalian sympathetic neurons. / Knoper, Steven R; Meehan, Alan G.; Purnyn, Sergey; Coggan, Jay S.; Anthony, Todd L.; Kreulen, David L.

In: European Journal of Pharmacology, Vol. 232, No. 1, 23.02.1993, p. 65-69.

Research output: Contribution to journalArticle

Knoper, Steven R ; Meehan, Alan G. ; Purnyn, Sergey ; Coggan, Jay S. ; Anthony, Todd L. ; Kreulen, David L. / CCKA receptors mediate slow depolarizations in cultured mammalian sympathetic neurons. In: European Journal of Pharmacology. 1993 ; Vol. 232, No. 1. pp. 65-69.
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