Abstract
CTL combat intracellular pathogens by killing infected cells. The molecular targets of their attack are foreign peptides bound to self MHC encoded class I molecules. Immunization of mice with peptides containing CTL determinants was shown to elicit CD4-dependent CTL. Here, we have achieved in vivo CTL priming with naturally processed 8-10 amino acid long class I-restricted peptides emulsified in an adjuvant. A potent, reproducible and physiologically relevant response was obtained using peptides from an intracellular bacterium and five viruses (including HIV) in two murine MHC haplotypes. This method is suitable for multiple vaccination, since a 'cocktail' of peptides derived from three pathogens elicited effector CTL against each pathogen. Most importantly, peptide-induced CD8+CD4- CTL were CD4+-independent These results have implications for CTL induction in situations where CD4 T cells are depleted or compromised, as is the case n HIV infection
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1205-1212 |
| Number of pages | 8 |
| Journal | International Immunology |
| Volume | 7 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1995 |
| Externally published | Yes |
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Keywords
- CD4
- CTL
- Peptide
- Vaccine
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Public Health, Environmental and Occupational Health
- Neuropsychology and Physiological Psychology
- Transplantation
- Immunology
Cite this
CD4-independent in vivo priming of murine CTL by optimal MHC class I-restricted peptides derived from intracellular pathogens. / Dyall, Rubendra; Vasović, Ljiljana V.; Molano, Alberto; Nikolich-Zugich, Janko.
In: International Immunology, Vol. 7, No. 8, 08.1995, p. 1205-1212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - CD4-independent in vivo priming of murine CTL by optimal MHC class I-restricted peptides derived from intracellular pathogens
AU - Dyall, Rubendra
AU - Vasović, Ljiljana V.
AU - Molano, Alberto
AU - Nikolich-Zugich, Janko
PY - 1995/8
Y1 - 1995/8
N2 - CTL combat intracellular pathogens by killing infected cells. The molecular targets of their attack are foreign peptides bound to self MHC encoded class I molecules. Immunization of mice with peptides containing CTL determinants was shown to elicit CD4-dependent CTL. Here, we have achieved in vivo CTL priming with naturally processed 8-10 amino acid long class I-restricted peptides emulsified in an adjuvant. A potent, reproducible and physiologically relevant response was obtained using peptides from an intracellular bacterium and five viruses (including HIV) in two murine MHC haplotypes. This method is suitable for multiple vaccination, since a 'cocktail' of peptides derived from three pathogens elicited effector CTL against each pathogen. Most importantly, peptide-induced CD8+CD4- CTL were CD4+-independent These results have implications for CTL induction in situations where CD4 T cells are depleted or compromised, as is the case n HIV infection
AB - CTL combat intracellular pathogens by killing infected cells. The molecular targets of their attack are foreign peptides bound to self MHC encoded class I molecules. Immunization of mice with peptides containing CTL determinants was shown to elicit CD4-dependent CTL. Here, we have achieved in vivo CTL priming with naturally processed 8-10 amino acid long class I-restricted peptides emulsified in an adjuvant. A potent, reproducible and physiologically relevant response was obtained using peptides from an intracellular bacterium and five viruses (including HIV) in two murine MHC haplotypes. This method is suitable for multiple vaccination, since a 'cocktail' of peptides derived from three pathogens elicited effector CTL against each pathogen. Most importantly, peptide-induced CD8+CD4- CTL were CD4+-independent These results have implications for CTL induction in situations where CD4 T cells are depleted or compromised, as is the case n HIV infection
KW - CD4
KW - CTL
KW - Peptide
KW - Vaccine
UR - http://www.scopus.com/inward/record.url?scp=0029102013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029102013&partnerID=8YFLogxK
U2 - 10.1093/intimm/7.8.1205
DO - 10.1093/intimm/7.8.1205
M3 - Article
C2 - 7495727
AN - SCOPUS:0029102013
VL - 7
SP - 1205
EP - 1212
JO - International Immunology
JF - International Immunology
SN - 0953-8178
IS - 8
ER -