CD4-independent in vivo priming of murine CTL by optimal MHC class I-restricted peptides derived from intracellular pathogens

Rubendra Dyall, Ljiljana V. Vasović, Alberto Molano, Janko Nikolich-Zugich

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

CTL combat intracellular pathogens by killing infected cells. The molecular targets of their attack are foreign peptides bound to self MHC encoded class I molecules. Immunization of mice with peptides containing CTL determinants was shown to elicit CD4-dependent CTL. Here, we have achieved in vivo CTL priming with naturally processed 8-10 amino acid long class I-restricted peptides emulsified in an adjuvant. A potent, reproducible and physiologically relevant response was obtained using peptides from an intracellular bacterium and five viruses (including HIV) in two murine MHC haplotypes. This method is suitable for multiple vaccination, since a 'cocktail' of peptides derived from three pathogens elicited effector CTL against each pathogen. Most importantly, peptide-induced CD8+CD4- CTL were CD4+-independent These results have implications for CTL induction in situations where CD4 T cells are depleted or compromised, as is the case n HIV infection

Original languageEnglish (US)
Pages (from-to)1205-1212
Number of pages8
JournalInternational Immunology
Volume7
Issue number8
DOIs
StatePublished - Aug 1995
Externally publishedYes

Fingerprint

Pathogens
Peptides
Immunization
HIV Infection
Haplotype
T-cells
Vaccination
Viruses
Bacteria
Haplotypes
Virus
HIV Infections
Amino Acids
Class
Priming
Amino acids
Mouse
Proof by induction
Determinant
Attack

Keywords

  • CD4
  • CTL
  • Peptide
  • Vaccine

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation
  • Immunology

Cite this

CD4-independent in vivo priming of murine CTL by optimal MHC class I-restricted peptides derived from intracellular pathogens. / Dyall, Rubendra; Vasović, Ljiljana V.; Molano, Alberto; Nikolich-Zugich, Janko.

In: International Immunology, Vol. 7, No. 8, 08.1995, p. 1205-1212.

Research output: Contribution to journalArticle

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