CD40 and IgE: Synergism between Anti-CD40 Monoclonal Antibody and Interleukin 4 in the Induction of IgE Synthesis by Highly Purified Human B Cells

Haifa H. Jabara, Shu Man Fu, Raif S. Geha, Donata Vercelli

Research output: Contribution to journalArticle

423 Scopus citations

Abstract

A novel pathway of IgE-B cell differentiation has been identified. Engagement of the B cell antigen CD40 by F(ab')2 fragments of monoclonal antibody (mAb) 626.1 in the presence of recombinant interleukin 4 (rIL4) induced intense IgE synthesis, but modest IgG synthesis, by highly purified human B cells. Surface IgE B cells isolated by cell sorting were induced to produce IgE by mAb 626.1 and IIr4. Thus, IgE synthesis is unlikely to result from expansion of a B cell population precommitted to IgE in vivo. A neutralizing anti-II76 antibody strongly, but not completely, inhibited the IgE response. This indicates that autocrine production of Il76 plays an important amplification role in IgE synthesis triggered by anti-CD40 mAb and 1174. Although the exact role played by CD40 in IgE responses in vivo remains to be established, this T cell-independent system represents a useful model to characterize the biochemical and molecular events leading to IgE synthesis in human B cells.

Original languageEnglish (US)
Pages (from-to)1861-1864
Number of pages4
JournalJournal of Experimental Medicine
Volume172
Issue number6
DOIs
StatePublished - Dec 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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