CD4+ cytolytic effecters are inefficient in the clearance of Listeria monocytogenes

J. S. Serody, R. M. Poston, D. Weinstock, R. J. Kurlander, Jeffrey A Frelinger

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cytotoxic T lymphocytes (CTL) recognize and lyse target cells through the interaction of the T-cell receptor complex with the class I or class II major histocompatibility complex (MHC). The production of class I-restricted CTL has been shown to be critical to the elimination of specific pathogens including Listeria monocytogenes. However, the function of class II-restricted CTL in the clearance of intracellular pathogens is poorly understood. H-2b β2-microglobulin-deficient mice (β2M-/-) are not able to produce CD8+ CTL in response to infection with L. monocytogenes. We used this model to evaluate the efficacy of class II-restricted CTL, in the absence of a class I-restricted response, during a primary infection with L. monocytogenes. We demonstrate that, despite their effectiveness in adoptive transfer of protection, Listeria-specific CD4+ class II-restricted cytotoxic lymphocytes are ineffective in decreasing titres of L. monocytogenes in the spleen after an established infection. In β2M-/- mice, persistence of L. monocytogenes in the spleen was found preferentially in class II-negative cells. Surprisingly, class I-restricted CTL from C57BL/6 mice were capable of decreasing bacterial titres during an established infection even in the absence of detectable class I on the surface of cells from β2M-/- mice. These data strongly suggest that, in the absence of a class I-restricted response, pathogens that elicit a class II-restricted cytotoxic response may escape prompt eradication by the immune system.

Original languageEnglish (US)
Pages (from-to)544-550
Number of pages7
JournalImmunology
Volume88
Issue number4
StatePublished - 1996
Externally publishedYes

Fingerprint

Listeria monocytogenes
Cytotoxic T-Lymphocytes
Infection
Spleen
Listeria
Adoptive Transfer
T-Cell Antigen Receptor
Major Histocompatibility Complex
Inbred C57BL Mouse
Cell Communication
Immune System
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Serody, J. S., Poston, R. M., Weinstock, D., Kurlander, R. J., & Frelinger, J. A. (1996). CD4+ cytolytic effecters are inefficient in the clearance of Listeria monocytogenes. Immunology, 88(4), 544-550.

CD4+ cytolytic effecters are inefficient in the clearance of Listeria monocytogenes. / Serody, J. S.; Poston, R. M.; Weinstock, D.; Kurlander, R. J.; Frelinger, Jeffrey A.

In: Immunology, Vol. 88, No. 4, 1996, p. 544-550.

Research output: Contribution to journalArticle

Serody, JS, Poston, RM, Weinstock, D, Kurlander, RJ & Frelinger, JA 1996, 'CD4+ cytolytic effecters are inefficient in the clearance of Listeria monocytogenes', Immunology, vol. 88, no. 4, pp. 544-550.
Serody JS, Poston RM, Weinstock D, Kurlander RJ, Frelinger JA. CD4+ cytolytic effecters are inefficient in the clearance of Listeria monocytogenes. Immunology. 1996;88(4):544-550.
Serody, J. S. ; Poston, R. M. ; Weinstock, D. ; Kurlander, R. J. ; Frelinger, Jeffrey A. / CD4+ cytolytic effecters are inefficient in the clearance of Listeria monocytogenes. In: Immunology. 1996 ; Vol. 88, No. 4. pp. 544-550.
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