CD8 T-cell immune phenotype of successful aging

Hui Chen Hsu, Donald K. Scott, Pili Zhang, Juling Zhou, PingAr Yang, Qi Wu, Harry W. Schroeder, Lynn B Gerald, Eric Ravussin, S. Michal Jazwinski, John D. Mountz, Mark Batzer, Meghan Black, Evest A. Broussard, Laurie Byerley, Pauline Callinan, Katie E. Cherry, Yu Wen Chiu, Annie Cooper, James P. DeLany & 24 others W. Andrew Deutsch, Elizabeth T H Fontham, Madlyn Frisard, Paula Geiselman, Valentina Greco, Karri S. Hawley, Scott W. Herke, S. Michal Jazwinski, Darla Kendzor, Sangkyu Kim, Beth Kimball, Li Li, Kay Lopez, Eric Ravussin, Yolanda Robertson, Henry Rothschild, Beth Schmidt, Donald K. Scott, F. Nicole Standberry, L. Joseph Su, Hui Yi Lin, Crystal P. Traylor, Robert H. Wood, Pili Zhang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The nonagenarian population by definition represents individuals who have demonstrated success in aging. We determined the status of CD8+ T-cell senescence in nonagenarians by analyzing the expression of CD28 and Fas (CD95), and analyzing activation and activation-induced cell death (AICD). Peripheral blood mononuclear cells (PBMCs) were isolated from three groups of subjects: adults (20-64-year-old), older adults (65-89-year-old), and nonagenarians (≥90-year-old). PBMCs were stimulated with phytohemagglutinin (PHA) (10 μg/ml). The cells were labeled with conjugated antibodies specific for CD4, CD8, CD28, CD45RO, and Fas, and were analyzed by FACS®. There was a strong negative correlation of the percentage of CD28 +Fas- CD8+ T-cells with the age of each individual prior to stimulation in vitro (R2 = 0.76, p < 0.0001). Compared to other biomarkers (CD28-, CD28-CD45RO +, and Fas+) that have been associated with CD8 + T-cell aging, the loss of the CD28+Fas- CD8+ T-cell population exhibited the strongest correlation with the individual's chronologic age. After stimulation with PHA, there was a decrease in the percentage of CD8+ T-cells from individual ≥65-year-old that expresses both CD28+ and Fas+ at day 3. Surprisingly, the AICD response of CD8+ T-cells at day 7 in the nonagenarians was higher than that in the other two groups. These results suggest that successful aging does not prevent development of the senescent phenotype of unstimulated CD8+ T cells, but is associated with preservation of CD8 T cell functions including activation and AICD. Increased AICD may result in enhanced rejuvenation capacity of T cells and limit the impact of aging on T cell function in nonagenarians.

Original languageEnglish (US)
Pages (from-to)231-239
Number of pages9
JournalMechanisms of Ageing and Development
Volume127
Issue number3
DOIs
StatePublished - Mar 2006
Externally publishedYes

Fingerprint

T-cells
Aging of materials
T-Lymphocytes
Phenotype
Chemical activation
Cell death
Cell Death
Cell Aging
Phytohemagglutinins
Blood Cells
Blood
Rejuvenation
Biomarkers
Population

Keywords

  • CD28
  • CD8 T-cells
  • Fas
  • Nonagenarians

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Hsu, H. C., Scott, D. K., Zhang, P., Zhou, J., Yang, P., Wu, Q., ... Zhang, P. (2006). CD8 T-cell immune phenotype of successful aging. Mechanisms of Ageing and Development, 127(3), 231-239. https://doi.org/10.1016/j.mad.2005.10.001

CD8 T-cell immune phenotype of successful aging. / Hsu, Hui Chen; Scott, Donald K.; Zhang, Pili; Zhou, Juling; Yang, PingAr; Wu, Qi; Schroeder, Harry W.; Gerald, Lynn B; Ravussin, Eric; Jazwinski, S. Michal; Mountz, John D.; Batzer, Mark; Black, Meghan; Broussard, Evest A.; Byerley, Laurie; Callinan, Pauline; Cherry, Katie E.; Chiu, Yu Wen; Cooper, Annie; DeLany, James P.; Deutsch, W. Andrew; Fontham, Elizabeth T H; Frisard, Madlyn; Geiselman, Paula; Greco, Valentina; Hawley, Karri S.; Herke, Scott W.; Jazwinski, S. Michal; Kendzor, Darla; Kim, Sangkyu; Kimball, Beth; Li, Li; Lopez, Kay; Ravussin, Eric; Robertson, Yolanda; Rothschild, Henry; Schmidt, Beth; Scott, Donald K.; Standberry, F. Nicole; Su, L. Joseph; Lin, Hui Yi; Traylor, Crystal P.; Wood, Robert H.; Zhang, Pili.

In: Mechanisms of Ageing and Development, Vol. 127, No. 3, 03.2006, p. 231-239.

Research output: Contribution to journalArticle

Hsu, HC, Scott, DK, Zhang, P, Zhou, J, Yang, P, Wu, Q, Schroeder, HW, Gerald, LB, Ravussin, E, Jazwinski, SM, Mountz, JD, Batzer, M, Black, M, Broussard, EA, Byerley, L, Callinan, P, Cherry, KE, Chiu, YW, Cooper, A, DeLany, JP, Deutsch, WA, Fontham, ETH, Frisard, M, Geiselman, P, Greco, V, Hawley, KS, Herke, SW, Jazwinski, SM, Kendzor, D, Kim, S, Kimball, B, Li, L, Lopez, K, Ravussin, E, Robertson, Y, Rothschild, H, Schmidt, B, Scott, DK, Standberry, FN, Su, LJ, Lin, HY, Traylor, CP, Wood, RH & Zhang, P 2006, 'CD8 T-cell immune phenotype of successful aging', Mechanisms of Ageing and Development, vol. 127, no. 3, pp. 231-239. https://doi.org/10.1016/j.mad.2005.10.001
Hsu HC, Scott DK, Zhang P, Zhou J, Yang P, Wu Q et al. CD8 T-cell immune phenotype of successful aging. Mechanisms of Ageing and Development. 2006 Mar;127(3):231-239. https://doi.org/10.1016/j.mad.2005.10.001
Hsu, Hui Chen ; Scott, Donald K. ; Zhang, Pili ; Zhou, Juling ; Yang, PingAr ; Wu, Qi ; Schroeder, Harry W. ; Gerald, Lynn B ; Ravussin, Eric ; Jazwinski, S. Michal ; Mountz, John D. ; Batzer, Mark ; Black, Meghan ; Broussard, Evest A. ; Byerley, Laurie ; Callinan, Pauline ; Cherry, Katie E. ; Chiu, Yu Wen ; Cooper, Annie ; DeLany, James P. ; Deutsch, W. Andrew ; Fontham, Elizabeth T H ; Frisard, Madlyn ; Geiselman, Paula ; Greco, Valentina ; Hawley, Karri S. ; Herke, Scott W. ; Jazwinski, S. Michal ; Kendzor, Darla ; Kim, Sangkyu ; Kimball, Beth ; Li, Li ; Lopez, Kay ; Ravussin, Eric ; Robertson, Yolanda ; Rothschild, Henry ; Schmidt, Beth ; Scott, Donald K. ; Standberry, F. Nicole ; Su, L. Joseph ; Lin, Hui Yi ; Traylor, Crystal P. ; Wood, Robert H. ; Zhang, Pili. / CD8 T-cell immune phenotype of successful aging. In: Mechanisms of Ageing and Development. 2006 ; Vol. 127, No. 3. pp. 231-239.
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AU - Scott, Donald K.

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AU - Zhou, Juling

AU - Yang, PingAr

AU - Wu, Qi

AU - Schroeder, Harry W.

AU - Gerald, Lynn B

AU - Ravussin, Eric

AU - Jazwinski, S. Michal

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AU - Batzer, Mark

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AU - Callinan, Pauline

AU - Cherry, Katie E.

AU - Chiu, Yu Wen

AU - Cooper, Annie

AU - DeLany, James P.

AU - Deutsch, W. Andrew

AU - Fontham, Elizabeth T H

AU - Frisard, Madlyn

AU - Geiselman, Paula

AU - Greco, Valentina

AU - Hawley, Karri S.

AU - Herke, Scott W.

AU - Jazwinski, S. Michal

AU - Kendzor, Darla

AU - Kim, Sangkyu

AU - Kimball, Beth

AU - Li, Li

AU - Lopez, Kay

AU - Ravussin, Eric

AU - Robertson, Yolanda

AU - Rothschild, Henry

AU - Schmidt, Beth

AU - Scott, Donald K.

AU - Standberry, F. Nicole

AU - Su, L. Joseph

AU - Lin, Hui Yi

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AU - Wood, Robert H.

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