CD8+ T cells express a T-helper 1-like phenotype after burn injury

Bruce A. Cairns, Rob Maile, Ian Buchanan, David Pilati, Suzan DeSerres, Edward J. Collins, Jeffrey A Frelinger, Anthony A. Meyer

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background. Previous studies suggest that CD8+ T cells are immunosuppressive after burn injury, but recent reports indicate that CD8+ T cells have several functions similar to CD4+ T cells, including the secretion of cytokines. This study uses HY male antigen in transgenic HY female mice to determine the antigen-specific response of activated CD8+ T cells after burn injury. Methods. HY TCR transgenic female mice underwent burn or sham injury. Seventy-two hours after the burn, splenocytes were stimulated with 20 μmol/L HY peptide for 16, 48, and 64 hours; cellular proliferation, intracellular interferon-γ and interleukin-2, and apoptosis were measured. Results. Burn injury significantly impaired proliferation to HY antigen (P ≤ .05). Activated CD8+ T cells from burned mice showed increased intracellular interferon-γ and interleukin-2 16 hours after stimulation compared with sham (P ≤ .05) and at no time was less than control mice. The percent of CD8+ T cells decreased with the time of stimulation but was not due to apoptosis by Annexin V staining. Conclusions. Activated CD8+ T cells express a TH1-like phenotype after burn injury. This provides evidence that CD8+ T cells are not simply suppressive and that is consistent with data that CD4+ T cells are primed for a TH1 response after burn injury.

Original languageEnglish (US)
Pages (from-to)210-216
Number of pages7
JournalSurgery
Volume130
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

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T-Lymphocytes
Phenotype
Wounds and Injuries
H-Y Antigen
Interferons
Interleukin-2
Interleukin-16
Apoptosis
Annexin A5
Immunosuppressive Agents
Transgenic Mice
Cell Proliferation
Staining and Labeling
Cytokines
Antigens

ASJC Scopus subject areas

  • Surgery

Cite this

Cairns, B. A., Maile, R., Buchanan, I., Pilati, D., DeSerres, S., Collins, E. J., ... Meyer, A. A. (2001). CD8+ T cells express a T-helper 1-like phenotype after burn injury. Surgery, 130(2), 210-216. https://doi.org/10.1067/msy.2001.115835

CD8+ T cells express a T-helper 1-like phenotype after burn injury. / Cairns, Bruce A.; Maile, Rob; Buchanan, Ian; Pilati, David; DeSerres, Suzan; Collins, Edward J.; Frelinger, Jeffrey A; Meyer, Anthony A.

In: Surgery, Vol. 130, No. 2, 2001, p. 210-216.

Research output: Contribution to journalArticle

Cairns, BA, Maile, R, Buchanan, I, Pilati, D, DeSerres, S, Collins, EJ, Frelinger, JA & Meyer, AA 2001, 'CD8+ T cells express a T-helper 1-like phenotype after burn injury', Surgery, vol. 130, no. 2, pp. 210-216. https://doi.org/10.1067/msy.2001.115835
Cairns BA, Maile R, Buchanan I, Pilati D, DeSerres S, Collins EJ et al. CD8+ T cells express a T-helper 1-like phenotype after burn injury. Surgery. 2001;130(2):210-216. https://doi.org/10.1067/msy.2001.115835
Cairns, Bruce A. ; Maile, Rob ; Buchanan, Ian ; Pilati, David ; DeSerres, Suzan ; Collins, Edward J. ; Frelinger, Jeffrey A ; Meyer, Anthony A. / CD8+ T cells express a T-helper 1-like phenotype after burn injury. In: Surgery. 2001 ; Vol. 130, No. 2. pp. 210-216.
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AB - Background. Previous studies suggest that CD8+ T cells are immunosuppressive after burn injury, but recent reports indicate that CD8+ T cells have several functions similar to CD4+ T cells, including the secretion of cytokines. This study uses HY male antigen in transgenic HY female mice to determine the antigen-specific response of activated CD8+ T cells after burn injury. Methods. HY TCR transgenic female mice underwent burn or sham injury. Seventy-two hours after the burn, splenocytes were stimulated with 20 μmol/L HY peptide for 16, 48, and 64 hours; cellular proliferation, intracellular interferon-γ and interleukin-2, and apoptosis were measured. Results. Burn injury significantly impaired proliferation to HY antigen (P ≤ .05). Activated CD8+ T cells from burned mice showed increased intracellular interferon-γ and interleukin-2 16 hours after stimulation compared with sham (P ≤ .05) and at no time was less than control mice. The percent of CD8+ T cells decreased with the time of stimulation but was not due to apoptosis by Annexin V staining. Conclusions. Activated CD8+ T cells express a TH1-like phenotype after burn injury. This provides evidence that CD8+ T cells are not simply suppressive and that is consistent with data that CD4+ T cells are primed for a TH1 response after burn injury.

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