CD8+ T cells express a T-helper 1-like phenotype after burn injury

Bruce A. Cairns, Rob Maile, Ian Buchanan, David Pilati, Suzan DeSerres, Edward J. Collins, Jeffrey A. Frelinger, Anthony A. Meyer

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Background. Previous studies suggest that CD8+ T cells are immunosuppressive after burn injury, but recent reports indicate that CD8+ T cells have several functions similar to CD4+ T cells, including the secretion of cytokines. This study uses HY male antigen in transgenic HY female mice to determine the antigen-specific response of activated CD8+ T cells after burn injury. Methods. HY TCR transgenic female mice underwent burn or sham injury. Seventy-two hours after the burn, splenocytes were stimulated with 20 μmol/L HY peptide for 16, 48, and 64 hours; cellular proliferation, intracellular interferon-γ and interleukin-2, and apoptosis were measured. Results. Burn injury significantly impaired proliferation to HY antigen (P ≤ .05). Activated CD8+ T cells from burned mice showed increased intracellular interferon-γ and interleukin-2 16 hours after stimulation compared with sham (P ≤ .05) and at no time was less than control mice. The percent of CD8+ T cells decreased with the time of stimulation but was not due to apoptosis by Annexin V staining. Conclusions. Activated CD8+ T cells express a TH1-like phenotype after burn injury. This provides evidence that CD8+ T cells are not simply suppressive and that is consistent with data that CD4+ T cells are primed for a TH1 response after burn injury.

Original languageEnglish (US)
Pages (from-to)210-216
Number of pages7
JournalSurgery
Volume130
Issue number2
DOIs
StatePublished - Jan 1 2001
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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    Cairns, B. A., Maile, R., Buchanan, I., Pilati, D., DeSerres, S., Collins, E. J., Frelinger, J. A., & Meyer, A. A. (2001). CD8+ T cells express a T-helper 1-like phenotype after burn injury. Surgery, 130(2), 210-216. https://doi.org/10.1067/msy.2001.115835