Cdk5-mediated phosphorylation regulates phosphatidylinositol 4-phosphate 5-kinase type i γ90 activity and cell invasion

Liqing Li, Tomasz Kolodziej, Naser Jafari, Jing Chen, Haining Zhu, Zenon Rajfur, Cai Huang

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Phosphatidylinositol 4-phosphate 5-kinase type I g (PIPKIγ90) regulates cell migration, invasion, and metastasis. However, it is unknown how cellular signals regulate those processes. Here, we show that cyclindependent kinase 5 (Cdk5), a protein kinase that regulates cell migration and invasion, phosphorylates PIPKIγ90 at S453, and that Cdk5-mediated PIPKIγ90 phosphorylation is essential for cell invasion. Moreover, Cdk5-mediated phosphorylation down-regulates the activity of PIPKIγ90 and the secretion of fibronectin, an extracellular matrix protein that regulates cell migration and invasion. Furthermore, inhibition of PIPKIg activity with the chemical inhibitor UNC3230 suppresses fibronectin secretion in a dose-dependent manner, whereas depletion of Cdk5 enhances fibronectin secretion. With total internal reflection fluorescence microscopy, we found that secreted fibronectin appears as round dots, which colocalize with Tks5 and CD9 but not with Zyxin. These data suggest that Cdk5-mediated PIPKIγ90 phosphorylation regulates cell invasion by controlling PIPKIγ90 activity and fibronectin secretion.

Original languageEnglish (US)
Pages (from-to)631-642
Number of pages12
JournalFASEB Journal
Volume33
Issue number1
DOIs
StatePublished - Jan 2019
Externally publishedYes

Keywords

  • Cell migration
  • Extracellular matrix protein
  • Fibronectin
  • Phosphatidylinositol kinase
  • Secretion

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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