cDNA array analysis of gene expression following hemorrhagic shock and resuscitation in rats

Hasan B. Alam, Svetlana Stegalkina, Peter M Rhee, Elena Koustova

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The aim of this study was to characterize gene expression following hemorrhagic shock and resuscitation with emphasis on the differences between various resuscitation strategies. Methods: Male Sprague Dawley rats (n=25; 5/group) were subjected to a three stage hemorrhage and resuscitated as follows: (1) sham hemorrhage; (2) sham resuscitation; (3) lactated Ringer's solution (LR), 3:1 volume; (4) 7.5% hypertonic saline (HTS) 9.7 ml/kg; (5) plasma, 1:1 volume. Liver, spleen, lung and muscle were collected 3 h post resuscitation and cDNA array analysis was performed on the total RNA. Results: Expression of 1176 genes was analyzed. Following resuscitation, 82 of the genes studied (7%) displayed an altered expression of at least 2-fold compared to the sham hemorrhage group. Depending on organ system under study and resuscitation conditions, expression of these 82 genes was down- or up-regulated, bringing the total number of expression alterations to 167. Largest number of organ-specific changes in gene expression was noted in liver (63/167), followed by lung (57), muscle (25), and spleen (22). Most of the resuscitation strategy specific changes were caused by plasma resuscitation (68/167), followed by LR (51), and HTS (48). In every organ studied, gene expression profile was dependent upon the fluid used for resuscitation. Conclusion: Cellular response to hemorrhagic shock, even at the level of gene expression, is dependent on the resuscitation strategy. We have discovered altered expression of genes not previously implicated in the physiology of hemorrhagic shock and resuscitation. Gene array technology provides a rapid and efficient means of dissecting the complex genetic regulation of cellular response to shock.

Original languageEnglish (US)
Pages (from-to)195-206
Number of pages12
JournalResuscitation
Volume54
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Hemorrhagic Shock
Oligonucleotide Array Sequence Analysis
Resuscitation
Gene Expression
Hemorrhage
Spleen
Muscles
Lung
Liver
Transcriptome
Genes
Sprague Dawley Rats
Shock
RNA
Technology

Keywords

  • Cellular injury
  • Functional genomics
  • Hemorrhage
  • Hypertonic saline
  • Liver
  • Lung
  • Muscle
  • Plasma
  • Rat
  • Resuscitative fluids
  • Spleen

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Nursing(all)

Cite this

cDNA array analysis of gene expression following hemorrhagic shock and resuscitation in rats. / Alam, Hasan B.; Stegalkina, Svetlana; Rhee, Peter M; Koustova, Elena.

In: Resuscitation, Vol. 54, No. 2, 2002, p. 195-206.

Research output: Contribution to journalArticle

Alam, Hasan B. ; Stegalkina, Svetlana ; Rhee, Peter M ; Koustova, Elena. / cDNA array analysis of gene expression following hemorrhagic shock and resuscitation in rats. In: Resuscitation. 2002 ; Vol. 54, No. 2. pp. 195-206.
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abstract = "The aim of this study was to characterize gene expression following hemorrhagic shock and resuscitation with emphasis on the differences between various resuscitation strategies. Methods: Male Sprague Dawley rats (n=25; 5/group) were subjected to a three stage hemorrhage and resuscitated as follows: (1) sham hemorrhage; (2) sham resuscitation; (3) lactated Ringer's solution (LR), 3:1 volume; (4) 7.5{\%} hypertonic saline (HTS) 9.7 ml/kg; (5) plasma, 1:1 volume. Liver, spleen, lung and muscle were collected 3 h post resuscitation and cDNA array analysis was performed on the total RNA. Results: Expression of 1176 genes was analyzed. Following resuscitation, 82 of the genes studied (7{\%}) displayed an altered expression of at least 2-fold compared to the sham hemorrhage group. Depending on organ system under study and resuscitation conditions, expression of these 82 genes was down- or up-regulated, bringing the total number of expression alterations to 167. Largest number of organ-specific changes in gene expression was noted in liver (63/167), followed by lung (57), muscle (25), and spleen (22). Most of the resuscitation strategy specific changes were caused by plasma resuscitation (68/167), followed by LR (51), and HTS (48). In every organ studied, gene expression profile was dependent upon the fluid used for resuscitation. Conclusion: Cellular response to hemorrhagic shock, even at the level of gene expression, is dependent on the resuscitation strategy. We have discovered altered expression of genes not previously implicated in the physiology of hemorrhagic shock and resuscitation. Gene array technology provides a rapid and efficient means of dissecting the complex genetic regulation of cellular response to shock.",
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N2 - The aim of this study was to characterize gene expression following hemorrhagic shock and resuscitation with emphasis on the differences between various resuscitation strategies. Methods: Male Sprague Dawley rats (n=25; 5/group) were subjected to a three stage hemorrhage and resuscitated as follows: (1) sham hemorrhage; (2) sham resuscitation; (3) lactated Ringer's solution (LR), 3:1 volume; (4) 7.5% hypertonic saline (HTS) 9.7 ml/kg; (5) plasma, 1:1 volume. Liver, spleen, lung and muscle were collected 3 h post resuscitation and cDNA array analysis was performed on the total RNA. Results: Expression of 1176 genes was analyzed. Following resuscitation, 82 of the genes studied (7%) displayed an altered expression of at least 2-fold compared to the sham hemorrhage group. Depending on organ system under study and resuscitation conditions, expression of these 82 genes was down- or up-regulated, bringing the total number of expression alterations to 167. Largest number of organ-specific changes in gene expression was noted in liver (63/167), followed by lung (57), muscle (25), and spleen (22). Most of the resuscitation strategy specific changes were caused by plasma resuscitation (68/167), followed by LR (51), and HTS (48). In every organ studied, gene expression profile was dependent upon the fluid used for resuscitation. Conclusion: Cellular response to hemorrhagic shock, even at the level of gene expression, is dependent on the resuscitation strategy. We have discovered altered expression of genes not previously implicated in the physiology of hemorrhagic shock and resuscitation. Gene array technology provides a rapid and efficient means of dissecting the complex genetic regulation of cellular response to shock.

AB - The aim of this study was to characterize gene expression following hemorrhagic shock and resuscitation with emphasis on the differences between various resuscitation strategies. Methods: Male Sprague Dawley rats (n=25; 5/group) were subjected to a three stage hemorrhage and resuscitated as follows: (1) sham hemorrhage; (2) sham resuscitation; (3) lactated Ringer's solution (LR), 3:1 volume; (4) 7.5% hypertonic saline (HTS) 9.7 ml/kg; (5) plasma, 1:1 volume. Liver, spleen, lung and muscle were collected 3 h post resuscitation and cDNA array analysis was performed on the total RNA. Results: Expression of 1176 genes was analyzed. Following resuscitation, 82 of the genes studied (7%) displayed an altered expression of at least 2-fold compared to the sham hemorrhage group. Depending on organ system under study and resuscitation conditions, expression of these 82 genes was down- or up-regulated, bringing the total number of expression alterations to 167. Largest number of organ-specific changes in gene expression was noted in liver (63/167), followed by lung (57), muscle (25), and spleen (22). Most of the resuscitation strategy specific changes were caused by plasma resuscitation (68/167), followed by LR (51), and HTS (48). In every organ studied, gene expression profile was dependent upon the fluid used for resuscitation. Conclusion: Cellular response to hemorrhagic shock, even at the level of gene expression, is dependent on the resuscitation strategy. We have discovered altered expression of genes not previously implicated in the physiology of hemorrhagic shock and resuscitation. Gene array technology provides a rapid and efficient means of dissecting the complex genetic regulation of cellular response to shock.

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KW - Hemorrhage

KW - Hypertonic saline

KW - Liver

KW - Lung

KW - Muscle

KW - Plasma

KW - Rat

KW - Resuscitative fluids

KW - Spleen

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