Cell biology

Incoherent feedforward control governs adaptation of activated ras in a eukaryotic chemotaxis pathway

Kosuke Takeda, Danying Shao, Micha Adler, Pascale G Charest, William F. Loomis, Herbert Levine, Alex Groisman, Wouter Jan Rappel, Richard A. Firtel

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

Adaptation in signaling systems, during which the output returns to a fixed baseline after a change in the input, often involves negative feedback loops and plays a crucial role in eukaryotic chemotaxis. We determined the dynamical response to a uniform change in chemoattractant concentration of a eukaryotic chemotaxis pathway immediately downstream from G protein - coupled receptors. The response of an activated Ras showed near-perfect adaptation, leading us to attempt to fit the results using mathematical models for the two possible simple network topologies that can provide perfect adaptation. Only the incoherent feedforward network accurately described the experimental results. This analysis revealed that adaptation in this Ras pathway is achieved through the proportional activation of upstream components and not through negative feedback loops. Furthermore, these results are consistent with a local excitation, global inhibition mechanism for gradient sensing, possibly with a Ras guanosine triphosphatase - activating protein acting as a global inhibitor.

Original languageEnglish (US)
Article numberra2
JournalScience Signaling
Volume5
Issue number205
DOIs
StatePublished - Jan 3 2012
Externally publishedYes

Fingerprint

Cytology
Feedforward control
Chemotaxis
Cell Biology
Feedback
Guanosine
Chemotactic Factors
G-Protein-Coupled Receptors
Theoretical Models
Chemical activation
Topology
Mathematical models
Proteins
Inhibition (Psychology)

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Cell biology : Incoherent feedforward control governs adaptation of activated ras in a eukaryotic chemotaxis pathway. / Takeda, Kosuke; Shao, Danying; Adler, Micha; Charest, Pascale G; Loomis, William F.; Levine, Herbert; Groisman, Alex; Rappel, Wouter Jan; Firtel, Richard A.

In: Science Signaling, Vol. 5, No. 205, ra2, 03.01.2012.

Research output: Contribution to journalArticle

Takeda, K, Shao, D, Adler, M, Charest, PG, Loomis, WF, Levine, H, Groisman, A, Rappel, WJ & Firtel, RA 2012, 'Cell biology: Incoherent feedforward control governs adaptation of activated ras in a eukaryotic chemotaxis pathway', Science Signaling, vol. 5, no. 205, ra2. https://doi.org/10.1126/scisignal.2002413
Takeda, Kosuke ; Shao, Danying ; Adler, Micha ; Charest, Pascale G ; Loomis, William F. ; Levine, Herbert ; Groisman, Alex ; Rappel, Wouter Jan ; Firtel, Richard A. / Cell biology : Incoherent feedforward control governs adaptation of activated ras in a eukaryotic chemotaxis pathway. In: Science Signaling. 2012 ; Vol. 5, No. 205.
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