Cell-Cell Communication in Heterocellular Cultures of Alveolar Epithelial Cells

Brant E. Isakson, Gregory J. Seedorf, Richard L. Lubman, W. Howard Evans, Scott A Boitano

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

The mammalian alveolar epithelium is composed of alveolar type I (AT1) and alveolar type II (AT2) cells that together coordinate tissue function. We used a heterocellular culture model of AT1 and AT2 cells to determine pathways for intercellular signaling between these two phenotypes. Gap junction protein (connexin) profiles of AT1 and AT2 cells in heterocellular cultures were similar to those seen in rat lung alveolar sections. Dye coupling studies revealed functional gap junctions between and among each cell phenotype. Localized mechanical stimulation resulted in propagated changes of intracellular Ca2+ to AT1 or AT2 cells independent of the stimulated cell phenotype. Ca2+ communication that originated after AT1 cell stimulation was inhibited by gap junction blockers, but not by an inhibitor of extracellular nucleotide signaling (apyrase). Conversely, Ca2+ communication after stimulation of AT2 cells was not significantly reduced by gap junction inhibitors. However, apyrase significantly reduced Ca2+ communication from AT2 to AT1 cells, but not from AT2 to AT2 cells. In conclusion, AT1 and AT2 cells have unique connexin profiles that allow for functional coupling and distinct intercellular pathways for coordination of Ca2+ signaling.

Original languageEnglish (US)
Pages (from-to)552-561
Number of pages10
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume29
Issue number5
DOIs
StatePublished - Nov 2003

Fingerprint

Alveolar Epithelial Cells
Connexins
Cell Communication
Apyrase
Communication
Gap Junctions
Rats
Coloring Agents
Nucleotides
Tissue
Phenotype
Epithelial Cells
Epithelium

ASJC Scopus subject areas

  • Cell Biology
  • Pulmonary and Respiratory Medicine
  • Molecular Biology

Cite this

Cell-Cell Communication in Heterocellular Cultures of Alveolar Epithelial Cells. / Isakson, Brant E.; Seedorf, Gregory J.; Lubman, Richard L.; Evans, W. Howard; Boitano, Scott A.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 29, No. 5, 11.2003, p. 552-561.

Research output: Contribution to journalArticle

Isakson, Brant E. ; Seedorf, Gregory J. ; Lubman, Richard L. ; Evans, W. Howard ; Boitano, Scott A. / Cell-Cell Communication in Heterocellular Cultures of Alveolar Epithelial Cells. In: American Journal of Respiratory Cell and Molecular Biology. 2003 ; Vol. 29, No. 5. pp. 552-561.
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