Cell culture approaches to understanding the actions of steroid hormones on the insect nervous system

Richard B. Levine, Jams C. Weeksb

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

During metamorphosis of the hawkmoth, Manduca sexta, ecdysteroids regulate the dendritic remodeling and programmed death of identified motoneurons. These changes contribute to the dramatic reorganization of behavior that accompanies metamorphosis. As a step toward elucidating cellular and molecular mechanisms by which ecdysteroids affect neuronal phenotype, we have investigated the responses of Manducamotoneurons to ecdysteroids in vitro. Following dendritic regression at the end of larval life, thoracic leg motoneurons placed in culture respond to ecdysteroids by an increase in branching complexity, similar to events in vivo. Growth cone structure is affected markedly by ecdysteroids. At pupation, a rise in ecdysteroids triggers the segment-specific death of proleg motoneurons: the same segmental pattern of death is observed when motoneurons from different segments are removed from the nervous system and exposed to ecdysteroids in vitro. These studies provide strong evidence that Manducamotoneurons are direct targets of steroid action and set the stage for further studies of the specific mechanisms involved.

Original languageEnglish (US)
Pages (from-to)73-86
Number of pages14
JournalDevelopmental Neuroscience
Volume18
Issue number1-2
DOIs
StatePublished - Jan 1996

Keywords

  • 20-Hydroxyecdysone
  • Manduca sexta
  • Metamorphosis

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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