Cell type-specific characterization of nuclear DNA contents within complex tissues and organs

Changqing Zhang, Fang C. Gong, Georgina M. Lambert, David W Galbraith

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Eukaryotic organisms are defined by the presence of a nucleus, which encloses the chromosomal DNA, and is characterized by its DNA content (C-value). Complex eukaryotic organisms contain organs and tissues that comprise interspersions of different cell types, within which polysomaty, endoreduplication, and cell cycle arrest is frequently observed. Little is known about the distribution of C-values across different cell types within these organs and tissues.Results: We have developed, and describe here, a method to precisely define the C-value status within any specific cell type within complex organs and tissues of plants. We illustrate the application of this method to Arabidopsis thaliana, specifically focusing on the different cell types found within the root.Conclusion: The method accurately and conveniently charts C-value within specific cell types, and provides novel insight into developmental processes. The method is, in principle, applicable to any transformable organism, including mammals, within which cell type specificity of regulation of endoreduplication, of polysomaty, and of cell cycle arrest is suspected.

Original languageEnglish (US)
Article number7
JournalPlant Methods
Volume1
Issue number1
DOIs
StatePublished - Oct 4 2005

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nuclear genome
DNA
Endoreduplication
endopolyploidy
cells
Cell Cycle Checkpoints
organisms
plant organs
Arabidopsis
application methods
plant tissues
tissues
Mammals
Arabidopsis thaliana
methodology
mammals
cell cycle checkpoints

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Plant Science

Cite this

Cell type-specific characterization of nuclear DNA contents within complex tissues and organs. / Zhang, Changqing; Gong, Fang C.; Lambert, Georgina M.; Galbraith, David W.

In: Plant Methods, Vol. 1, No. 1, 7, 04.10.2005.

Research output: Contribution to journalArticle

Zhang, Changqing ; Gong, Fang C. ; Lambert, Georgina M. ; Galbraith, David W. / Cell type-specific characterization of nuclear DNA contents within complex tissues and organs. In: Plant Methods. 2005 ; Vol. 1, No. 1.
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