Cellular analysis of the mode of action of methyl-3,5-diiodo-4-(4′-methoxyphenoxy) benzoate (DIME) on tumor cells

Julia Yuzhen, Burt Feuerstein, Charles A. Vidair, William C. Hyun, Lawrence T. Young, Eva Kirsten, Ernest Kun

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The hormonally inactive methyl-3,5-diiodo-4-(4′-methoxyphenoxy) benzoate (DIME) at 1-4 μM concentration induces morphologic changes in E-ras 20 and MDA-MB-231 and other human cancer cells such as multinucleation and enlargement and arrests the cell cycle in the M phase without affecting interphase. Time-lapse videomicroscopy allowed us to follow individual cells. Cells exposed to DIME divided in an abnormal manner, leading to 20% cell fusion and multinucleation. Chromosome painting demonstrated a large accumulation of chromosomes after 5 days of treatment with DIME, consistent with the failure of cells to divide normally. Chromosome breakage was not observed. On the other hand, highly abnormal tubulin-containing structures ensue upon exposure to DIME (1 μM for 18 h) treatment, indicating an early biochemical action of DIME on the spindle assembly system.

Original languageEnglish (US)
Pages (from-to)905-910
Number of pages6
JournalInternational journal of oncology
Volume10
Issue number5
StatePublished - Dec 1 1997
Externally publishedYes

Keywords

  • Cell division
  • Flow cytometry
  • Time-lapse videomicroscopy
  • Tubulin

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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