Cellular composition and steroidogenic capacity of the ovary of Macrotus californicus (Chiroptera: Phyllostomatidae) during and after delayed embryonic development

Elizabeth G. Crichton, Patricia B Hoyer, Philip H. Krutzsch

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In the leaf-nosed bat, Macrotus californicus, a 4,5-month period of delayed early embryogenesis (October-March) precedes a 3.5-month period of normal embryogenesis (March-June). This prolonged gestation provides a unique opportunity to correlate ovarian changes with the events following implantation. The present study investigated luteal cell development and follicular biology during gestation. Circulating progesterone (P) levels following implantation were unchanged before transition to normal development, and were maximal at the start of active gestation. Luteal cell diameters increased during this period. Serum P levels declined prior to parturition, when cells staining positive for 3β-hydroxy-5-steroid dehydrogenase-5,4-isomerase (3β-HSD) activity were reduced in number and diameter, and enzyme staining was less intense in tissue slices. Subcellular steroidogenic organelles were present during delayed development, but smooth endoplasmic reticulum (SER) was markedly increased after the resumption of normal development at which time also luteal cells reacted positively to staining for 17β-HSD. Before parturition, lipid droplet accumulation and reduced SER suggested a reduction in steroid secretion. Large multilaminar follicles stained positive for 3β-HSD activity throughout gestation and for 17β-HSD except in late delayed development. Thus, the delay in embryogenesis may be due to an inadequately developed corpus luteum or to the steroidogenic activity of the multilaminar follicles.

Original languageEnglish (US)
Pages (from-to)355-366
Number of pages12
JournalCell & Tissue Research
Volume260
Issue number2
DOIs
StatePublished - May 1990

Fingerprint

Luteal Cells
Embryonic Development
Ovary
Steroids
Smooth Endoplasmic Reticulum
Pregnancy
Isomerases
Staining and Labeling
Chemical analysis
Progesterone
Oxidoreductases
Parturition
Tissue
Lipids
Corpus Luteum
Enzymes
Organelles
Serum

Keywords

  • Corpus luteum
  • Delayed development
  • Macrotus californicus (Chiroptera)
  • Multilaminar follicles
  • Steroidogenesis

ASJC Scopus subject areas

  • Anatomy
  • Clinical Biochemistry
  • Cell Biology

Cite this

Cellular composition and steroidogenic capacity of the ovary of Macrotus californicus (Chiroptera : Phyllostomatidae) during and after delayed embryonic development. / Crichton, Elizabeth G.; Hoyer, Patricia B; Krutzsch, Philip H.

In: Cell & Tissue Research, Vol. 260, No. 2, 05.1990, p. 355-366.

Research output: Contribution to journalArticle

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