Cellular Origin and Rate of Endothelial Cell Coverage of PTFE Grafts

David A. Bull, Glenn C. Hunter, Hana Holubec, William - Adamas-Rappaport, William D. Rappaport, Charles W. Putnam

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To determine the origin, cell type present, and rate of endothelial cell coverage of PTFE grafts, 5-cm segments of 4-mm-diameter, 60-μm PTFE grafts were implanted end-to-end bilaterally in the carotid arteries of greyhound dogs, An external jugular vein wrap was applied to the outer surface of one of the PTFE grafts; the contralateral PTFE graft, which was unwrapped, served as its control. Two dogs each were sacrificed at 3, 5, 7, 14, 21, 28, and 35 days postimplantation. Anastomotic endothelial ingrowth was analyzed using scanning electron microscopy. Microvessel ingrowth was documented in longitudinal H&E sections. Cell identity was established by immunohistochemistry with factor VIII antibody, Ulex europaes, leukocyte common antigen, and antibodies to α-actin, desmin, vimentin, and basic fibroblast growth factor. All grafts were patent at the time of harvest. Endothelial cell migration from the native artery adjacent to the anastomosis commenced at 7 days, extended to 5 mm beyond the proximal and distal anastomoses by 14 days and to 1.0 cm by 35 days. Endothelialization of the mid-portion of the wrapped grafts occurred via microvessel ingrowth, a process which began at 7 days. Microvessels reached the luminal surface by 28 days and an endothelial cell monolayer was established by 35 days. Wrapping the external surface of the graft with vein increased the rate of graft healing. Basic fibroblast growth factor was detectable by immunohistochemistry at the vein wrap-graft interface in the first 14 days.

Original languageEnglish (US)
Pages (from-to)58-68
Number of pages11
JournalJournal of Surgical Research
Volume58
Issue number1
DOIs
StatePublished - Jan 1995

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Polytetrafluoroethylene
Endothelial Cells
Transplants
Microvessels
Fibroblast Growth Factor 2
Veins
Immunohistochemistry
Dogs
Ulex
CD45 Antigens
Desmin
Antibodies
Jugular Veins
Factor VIII
Vimentin
Carotid Arteries
Electron Scanning Microscopy
Cell Movement
Actins
Arteries

ASJC Scopus subject areas

  • Surgery

Cite this

Cellular Origin and Rate of Endothelial Cell Coverage of PTFE Grafts. / Bull, David A.; Hunter, Glenn C.; Holubec, Hana; Adamas-Rappaport, William -; Rappaport, William D.; Putnam, Charles W.

In: Journal of Surgical Research, Vol. 58, No. 1, 01.1995, p. 58-68.

Research output: Contribution to journalArticle

Bull, David A. ; Hunter, Glenn C. ; Holubec, Hana ; Adamas-Rappaport, William - ; Rappaport, William D. ; Putnam, Charles W. / Cellular Origin and Rate of Endothelial Cell Coverage of PTFE Grafts. In: Journal of Surgical Research. 1995 ; Vol. 58, No. 1. pp. 58-68.
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