Cellular resolution multiplexed FLIM tomography with dual-color Bessel beam

Dongli Xu, Weibin Zhou, Leilei Peng

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Fourier multiplexed FLIM (FmFLIM) tomography enables multiplexed 3D lifetime imaging of whole embryos. In our previous FmFLIM system, the spatial resolution was limited to 25 μm because of the trade-off between the spatial resolution and the imaging depth. In order to achieve cellular resolution imaging of thick specimens, we built a tomography system with dual-color Bessel beam. In combination with FmFLIM, the Bessel FmFLIM tomography system can perform parallel 3D lifetime imaging on multiple excitation-emission channels at a cellular resolution of 2.8 μm. The image capability of the Bessel FmFLIM tomography system was demonstrated by 3D lifetime imaging of dual-labeled transgenic zebrafish embryos.

Original languageEnglish (US)
Article number#275798
Pages (from-to)570-578
Number of pages9
JournalBiomedical Optics Express
Volume8
Issue number2
DOIs
StatePublished - Feb 1 2017

Keywords

  • Fluorescence microscopy
  • Imaging systems
  • Spectroscopy, fluorescence and luminescence
  • Three-dimensional microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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