Cellular volume regulation in freshwater bivalves

T. H. Dietz, D. H. Neufeld, H. Silverman, Stephen Wright

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effect of ambient osmolality on the height of lateral ciliated cells from the gills of two freshwater bivalve species (Dreissena polymorpha, Toxolasma texasensis) was directly observed microscopically. The addition of 1 mmol·l-1 KCl to an artificial pondwater (APW) superfusion medium resulted in an increase in cell height. When the superfusion solution was made hyperosmotic (~90 mmol·kg-1 H2O) by the addition of 45 mmol·l-1 NaCl to APW, the cell height decreased by about 20-30% and there was no evidence of a regulatory volume increase over 20-30 min. In contrast, when 1 mmol·l-1 KCl was added to the hyperosmotic medium the cell height always partially (40-50%) recovered. When the gill tissue was returned to APW following the hyperosmotic treatment the cells returned to the original cell height. Bivalve gills superfused with the hyperosmotic NaCl and KCl solution in the presence of 1 mmol·l-1 ouabain experienced a similar 25% decrease in cell height. When the ouabain-treated tissues were returned to APW the cells swelled, overshooting the original cell height. These results indicate these fresh-water bivalves have a limited ability for cellular volume regulation using inorganic ions, but depend on a suitable balance of Na+ and K+ in the environment to effect regulatory volume changes.

Original languageEnglish (US)
Pages (from-to)87-95
Number of pages9
JournalJournal of Comparative Physiology B
Volume168
Issue number2
DOIs
StatePublished - 1998

Fingerprint

Bivalvia
Ouabain
Fresh Water
bivalve
Tissue
Artificial Cells
gills
cells
Ions
ouabain
Water
Dreissena
inorganic ions
Dreissena polymorpha
volume change
osmolality
regulation
Osmolar Concentration
ion

Keywords

  • Dreissena polymorpha
  • Freshwater bivalve
  • K-dependent cellular volume regulation
  • Ouabain
  • Toxolasma texasensis

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Environmental Science(all)
  • Physiology
  • Physiology (medical)

Cite this

Cellular volume regulation in freshwater bivalves. / Dietz, T. H.; Neufeld, D. H.; Silverman, H.; Wright, Stephen.

In: Journal of Comparative Physiology B, Vol. 168, No. 2, 1998, p. 87-95.

Research output: Contribution to journalArticle

Dietz, T. H. ; Neufeld, D. H. ; Silverman, H. ; Wright, Stephen. / Cellular volume regulation in freshwater bivalves. In: Journal of Comparative Physiology B. 1998 ; Vol. 168, No. 2. pp. 87-95.
@article{047b8767b8b54772908b3ad0b4129d91,
title = "Cellular volume regulation in freshwater bivalves",
abstract = "The effect of ambient osmolality on the height of lateral ciliated cells from the gills of two freshwater bivalve species (Dreissena polymorpha, Toxolasma texasensis) was directly observed microscopically. The addition of 1 mmol·l-1 KCl to an artificial pondwater (APW) superfusion medium resulted in an increase in cell height. When the superfusion solution was made hyperosmotic (~90 mmol·kg-1 H2O) by the addition of 45 mmol·l-1 NaCl to APW, the cell height decreased by about 20-30{\%} and there was no evidence of a regulatory volume increase over 20-30 min. In contrast, when 1 mmol·l-1 KCl was added to the hyperosmotic medium the cell height always partially (40-50{\%}) recovered. When the gill tissue was returned to APW following the hyperosmotic treatment the cells returned to the original cell height. Bivalve gills superfused with the hyperosmotic NaCl and KCl solution in the presence of 1 mmol·l-1 ouabain experienced a similar 25{\%} decrease in cell height. When the ouabain-treated tissues were returned to APW the cells swelled, overshooting the original cell height. These results indicate these fresh-water bivalves have a limited ability for cellular volume regulation using inorganic ions, but depend on a suitable balance of Na+ and K+ in the environment to effect regulatory volume changes.",
keywords = "Dreissena polymorpha, Freshwater bivalve, K-dependent cellular volume regulation, Ouabain, Toxolasma texasensis",
author = "Dietz, {T. H.} and Neufeld, {D. H.} and H. Silverman and Stephen Wright",
year = "1998",
doi = "10.1007/s003600050124",
language = "English (US)",
volume = "168",
pages = "87--95",
journal = "Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology",
issn = "0174-1578",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Cellular volume regulation in freshwater bivalves

AU - Dietz, T. H.

AU - Neufeld, D. H.

AU - Silverman, H.

AU - Wright, Stephen

PY - 1998

Y1 - 1998

N2 - The effect of ambient osmolality on the height of lateral ciliated cells from the gills of two freshwater bivalve species (Dreissena polymorpha, Toxolasma texasensis) was directly observed microscopically. The addition of 1 mmol·l-1 KCl to an artificial pondwater (APW) superfusion medium resulted in an increase in cell height. When the superfusion solution was made hyperosmotic (~90 mmol·kg-1 H2O) by the addition of 45 mmol·l-1 NaCl to APW, the cell height decreased by about 20-30% and there was no evidence of a regulatory volume increase over 20-30 min. In contrast, when 1 mmol·l-1 KCl was added to the hyperosmotic medium the cell height always partially (40-50%) recovered. When the gill tissue was returned to APW following the hyperosmotic treatment the cells returned to the original cell height. Bivalve gills superfused with the hyperosmotic NaCl and KCl solution in the presence of 1 mmol·l-1 ouabain experienced a similar 25% decrease in cell height. When the ouabain-treated tissues were returned to APW the cells swelled, overshooting the original cell height. These results indicate these fresh-water bivalves have a limited ability for cellular volume regulation using inorganic ions, but depend on a suitable balance of Na+ and K+ in the environment to effect regulatory volume changes.

AB - The effect of ambient osmolality on the height of lateral ciliated cells from the gills of two freshwater bivalve species (Dreissena polymorpha, Toxolasma texasensis) was directly observed microscopically. The addition of 1 mmol·l-1 KCl to an artificial pondwater (APW) superfusion medium resulted in an increase in cell height. When the superfusion solution was made hyperosmotic (~90 mmol·kg-1 H2O) by the addition of 45 mmol·l-1 NaCl to APW, the cell height decreased by about 20-30% and there was no evidence of a regulatory volume increase over 20-30 min. In contrast, when 1 mmol·l-1 KCl was added to the hyperosmotic medium the cell height always partially (40-50%) recovered. When the gill tissue was returned to APW following the hyperosmotic treatment the cells returned to the original cell height. Bivalve gills superfused with the hyperosmotic NaCl and KCl solution in the presence of 1 mmol·l-1 ouabain experienced a similar 25% decrease in cell height. When the ouabain-treated tissues were returned to APW the cells swelled, overshooting the original cell height. These results indicate these fresh-water bivalves have a limited ability for cellular volume regulation using inorganic ions, but depend on a suitable balance of Na+ and K+ in the environment to effect regulatory volume changes.

KW - Dreissena polymorpha

KW - Freshwater bivalve

KW - K-dependent cellular volume regulation

KW - Ouabain

KW - Toxolasma texasensis

UR - http://www.scopus.com/inward/record.url?scp=0031921490&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031921490&partnerID=8YFLogxK

U2 - 10.1007/s003600050124

DO - 10.1007/s003600050124

M3 - Article

VL - 168

SP - 87

EP - 95

JO - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

JF - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology

SN - 0174-1578

IS - 2

ER -