CFU-GM content of bone marrow graft correlates with time to hematologic reconstitution following autologous bone marrow transplantation with 4-hydroperoxycyclophosphamide-purged bone marrow

S. D. Rowley, M. Zuehlsdorf, H. G. Braine, O. M. Colvin, J. Davis, R. J. Jones, R. Saral, L. L. Sensenbrenner, Andrew M Yeager, G. W. Santos

Research output: Contribution to journalArticle

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Abstract

Autologous bone marrow transplants (BMTs) can repopulate the hematologic system of patients treated with marrow-ablative chemotherapy and/or radiotherapy. However, treatment of the bone marrow graft to eliminate residual tumor cells prior to reinfusion can delay the return of peripheral blood elements, presumably from damage to or loss of hematopoietic stem cells responsible for hematologic recovery. To develop a model predictive of hematologic recovery, we studied the progenitor cell contents of 4-hydroperoxycyclophosphamide (100 μg/mL)-purged bone marrow grafts of 40 consecutive patients undergoing autologous BMT at this center. Granulocyte-macrophage colonies (CFU-GM) were grown from all grafts after treatment with this chemotherapeutic agent, but erythroid (BFU-E) and mixed (CFU-GEMM) colonies were grown from only 44% and 33% of the grafts respectively. The recovery of CFU-GM after purging ranged from 0.07% to 23%. The logarithm of CFU-GM content of the treated grafts was linearly correlated with the time to recovery of peripheral blood leukocytes (r = -0.80), neutrophils (r = -0.79), reticulocytes (r = -0.60), and platelets (r = -0.66). The CFU-GM content of purged autologous bone marrow grafts may reflect the hematopoietic stem cell content of the grafts and thus predict the rate of hematologic recovery in patients undergoing autologous BMT.

Original languageEnglish (US)
Pages (from-to)271-275
Number of pages5
JournalBlood
Volume70
Issue number1
StatePublished - 1987
Externally publishedYes

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perfosfamide
Granulocyte-Macrophage Progenitor Cells
Autologous Transplantation
Bone Marrow Transplantation
Grafts
Bone
Bone Marrow
Transplants
Recovery
Stem cells
Blood
Hematopoietic Stem Cells
Purging
Chemotherapy
Macrophages
Radiotherapy
Platelets
Myeloid Progenitor Cells
Erythroid Precursor Cells
Tumors

ASJC Scopus subject areas

  • Hematology

Cite this

Rowley, S. D., Zuehlsdorf, M., Braine, H. G., Colvin, O. M., Davis, J., Jones, R. J., ... Santos, G. W. (1987). CFU-GM content of bone marrow graft correlates with time to hematologic reconstitution following autologous bone marrow transplantation with 4-hydroperoxycyclophosphamide-purged bone marrow. Blood, 70(1), 271-275.

CFU-GM content of bone marrow graft correlates with time to hematologic reconstitution following autologous bone marrow transplantation with 4-hydroperoxycyclophosphamide-purged bone marrow. / Rowley, S. D.; Zuehlsdorf, M.; Braine, H. G.; Colvin, O. M.; Davis, J.; Jones, R. J.; Saral, R.; Sensenbrenner, L. L.; Yeager, Andrew M; Santos, G. W.

In: Blood, Vol. 70, No. 1, 1987, p. 271-275.

Research output: Contribution to journalArticle

Rowley, SD, Zuehlsdorf, M, Braine, HG, Colvin, OM, Davis, J, Jones, RJ, Saral, R, Sensenbrenner, LL, Yeager, AM & Santos, GW 1987, 'CFU-GM content of bone marrow graft correlates with time to hematologic reconstitution following autologous bone marrow transplantation with 4-hydroperoxycyclophosphamide-purged bone marrow', Blood, vol. 70, no. 1, pp. 271-275.
Rowley, S. D. ; Zuehlsdorf, M. ; Braine, H. G. ; Colvin, O. M. ; Davis, J. ; Jones, R. J. ; Saral, R. ; Sensenbrenner, L. L. ; Yeager, Andrew M ; Santos, G. W. / CFU-GM content of bone marrow graft correlates with time to hematologic reconstitution following autologous bone marrow transplantation with 4-hydroperoxycyclophosphamide-purged bone marrow. In: Blood. 1987 ; Vol. 70, No. 1. pp. 271-275.
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abstract = "Autologous bone marrow transplants (BMTs) can repopulate the hematologic system of patients treated with marrow-ablative chemotherapy and/or radiotherapy. However, treatment of the bone marrow graft to eliminate residual tumor cells prior to reinfusion can delay the return of peripheral blood elements, presumably from damage to or loss of hematopoietic stem cells responsible for hematologic recovery. To develop a model predictive of hematologic recovery, we studied the progenitor cell contents of 4-hydroperoxycyclophosphamide (100 μg/mL)-purged bone marrow grafts of 40 consecutive patients undergoing autologous BMT at this center. Granulocyte-macrophage colonies (CFU-GM) were grown from all grafts after treatment with this chemotherapeutic agent, but erythroid (BFU-E) and mixed (CFU-GEMM) colonies were grown from only 44{\%} and 33{\%} of the grafts respectively. The recovery of CFU-GM after purging ranged from 0.07{\%} to 23{\%}. The logarithm of CFU-GM content of the treated grafts was linearly correlated with the time to recovery of peripheral blood leukocytes (r = -0.80), neutrophils (r = -0.79), reticulocytes (r = -0.60), and platelets (r = -0.66). The CFU-GM content of purged autologous bone marrow grafts may reflect the hematopoietic stem cell content of the grafts and thus predict the rate of hematologic recovery in patients undergoing autologous BMT.",
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AU - Braine, H. G.

AU - Colvin, O. M.

AU - Davis, J.

AU - Jones, R. J.

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AU - Sensenbrenner, L. L.

AU - Yeager, Andrew M

AU - Santos, G. W.

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AB - Autologous bone marrow transplants (BMTs) can repopulate the hematologic system of patients treated with marrow-ablative chemotherapy and/or radiotherapy. However, treatment of the bone marrow graft to eliminate residual tumor cells prior to reinfusion can delay the return of peripheral blood elements, presumably from damage to or loss of hematopoietic stem cells responsible for hematologic recovery. To develop a model predictive of hematologic recovery, we studied the progenitor cell contents of 4-hydroperoxycyclophosphamide (100 μg/mL)-purged bone marrow grafts of 40 consecutive patients undergoing autologous BMT at this center. Granulocyte-macrophage colonies (CFU-GM) were grown from all grafts after treatment with this chemotherapeutic agent, but erythroid (BFU-E) and mixed (CFU-GEMM) colonies were grown from only 44% and 33% of the grafts respectively. The recovery of CFU-GM after purging ranged from 0.07% to 23%. The logarithm of CFU-GM content of the treated grafts was linearly correlated with the time to recovery of peripheral blood leukocytes (r = -0.80), neutrophils (r = -0.79), reticulocytes (r = -0.60), and platelets (r = -0.66). The CFU-GM content of purged autologous bone marrow grafts may reflect the hematopoietic stem cell content of the grafts and thus predict the rate of hematologic recovery in patients undergoing autologous BMT.

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