TY - JOUR
T1 - Chalcone isomerase gene from rice (Oryza sativa) and barley (Hordeum vulgare)
T2 - Physical, genetic and mutation mapping
AU - Druka, Arnis
AU - Kudrna, David
AU - Rostoks, Nils
AU - Brueggeman, Robert
AU - Von Wettstein, Diter
AU - Kleinhofs, Andris
N1 - Funding Information:
This is Scientific Paper No. 10020 from the College of Agriculture and Home Economics Research Center, Washington State University, Pullman, WA; Project 99-35300-7694. Research was supported by USDA/NRI Grant No. 9600794 to A. K. Many thanks to Dr Takuji Sasaki for the EST clone S16470, Dr Erich Grotewold for the maize clone ZmCHI1, Dr Joseph Noel and Dr Gottfried Weissenböck for helpful personal communications.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2003/1/2
Y1 - 2003/1/2
N2 - The barley and rice chalcone flavonone isomerase (Cfi) genes were isolated and identified by homology to the maize Cfi gene. Structure analysis indicated high similarity except that the barley gene lacked intron 3. The maize Cfi gene has been mapped to three loci, but only a single locus was detected in barley and rice. This explains the lack of observed mutants in maize while a single locus anthocyanin-less 30 (ant30), with four alleles ant30-245, ant30-310, ant30-272 and ant30-287 has been described in barley. Based on biochemical analysis it has been suggested that these mutants are in the Cfi gene resulting in absence of anthocyanin. In order to provide molecular evidence for or against this hypothesis we sequenced the four ant30 alleles and compared them to their respective wild-type alleles. The three sodium azide induced mutants ant30-245, ant30-272 and ant30-287 showed single base changes resulting in two non-sense and one mis-sense mutations affecting the protein function. The 1-nitroso-5,6-dihydrouracil induced mutant ant30-310 had one base substitution and a 25 bp deletion. These observations are in accordance with the conclusion that the ant30 phenotype is caused by mutations in the Cfi gene. The nature of the mutants induced is in line with the proposed mechanism of action for the mutagens used.
AB - The barley and rice chalcone flavonone isomerase (Cfi) genes were isolated and identified by homology to the maize Cfi gene. Structure analysis indicated high similarity except that the barley gene lacked intron 3. The maize Cfi gene has been mapped to three loci, but only a single locus was detected in barley and rice. This explains the lack of observed mutants in maize while a single locus anthocyanin-less 30 (ant30), with four alleles ant30-245, ant30-310, ant30-272 and ant30-287 has been described in barley. Based on biochemical analysis it has been suggested that these mutants are in the Cfi gene resulting in absence of anthocyanin. In order to provide molecular evidence for or against this hypothesis we sequenced the four ant30 alleles and compared them to their respective wild-type alleles. The three sodium azide induced mutants ant30-245, ant30-272 and ant30-287 showed single base changes resulting in two non-sense and one mis-sense mutations affecting the protein function. The 1-nitroso-5,6-dihydrouracil induced mutant ant30-310 had one base substitution and a 25 bp deletion. These observations are in accordance with the conclusion that the ant30 phenotype is caused by mutations in the Cfi gene. The nature of the mutants induced is in line with the proposed mechanism of action for the mutagens used.
KW - Anthocyanin-less 30
KW - Azide mutagenesis
KW - Flavonoids
KW - Synteny
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U2 - 10.1016/S0378-1119(02)01105-8
DO - 10.1016/S0378-1119(02)01105-8
M3 - Article
C2 - 12527208
AN - SCOPUS:0037413671
VL - 302
SP - 171
EP - 178
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1-2
ER -