Changes in Na,K-ATPase gene expression during granulocytic differentiation of HL60 cells

Setsuko K Chambers, Maureen Gilmore-Hebert, Barry M. Kacinski, Edward J. Benz

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

During granulocytic differentiation of HL60 cells, immediate reduction of ouabain-sensitive potassium flux is observed within the first 12 hours of addition of dimethyl sulfoxide (DMSO). We show that gene expression of the α3 isoform of Na+,K+-ATPase, which encodes an ouabain-inhibitable Na+,K+-ATPase activity, significantly declines during the first 24 hours of granulocytic differentiation by DMSO of HL60 cells. The more common α1 isoform decreases, but more gradually over 72 hours of DMSO induction. Loss of α3 and α1 messenger RNA (mRNA) are due to changes in mRNA decay; their transcription is not altered. α3 mRNA half-life is 3 hours in HL60 cells; upon induction by 16 hours of DMSO, it decreases to approximately 2 hours. α1 transcripts are less sensitive to DMSO induction, with their half-life being 3.5 hours in HL60 cells; upon induction, their half-life decreases to 3 hours. Experiments measuring protein stability confirm that α3 protein is more labile than α1. In uninduced HL60 cells, α3 membrane protein comprises 30% of the total α isoforms, and is less stable than α1, with a protein half-life of only 9 hours. Upon DMSO induction, steady-state α3 protein decreases markedly within 10 hours, whereas α1 protein remains stable. These results show that posttranscriptional changes during induction play a major role in the differential regulation of α1 and α3 isoforms of Na+,K+-ATPase; regulation of the latter may be important for early granulocytic differentiation, or for one of the differentiated functions of mature granulocytes.

Original languageEnglish (US)
Pages (from-to)1559-1564
Number of pages6
JournalBlood
Volume80
Issue number6
StatePublished - Sep 15 1992
Externally publishedYes

Fingerprint

HL-60 Cells
Dimethyl Sulfoxide
Gene expression
Adenosine Triphosphatases
Gene Expression
Half-Life
Protein Isoforms
Ouabain
Proteins
Messenger RNA
Protein Stability
RNA Stability
Transcription
Granulocytes
sodium-translocating ATPase
Potassium
Membrane Proteins
Fluxes
Experiments

ASJC Scopus subject areas

  • Hematology

Cite this

Chambers, S. K., Gilmore-Hebert, M., Kacinski, B. M., & Benz, E. J. (1992). Changes in Na,K-ATPase gene expression during granulocytic differentiation of HL60 cells. Blood, 80(6), 1559-1564.

Changes in Na,K-ATPase gene expression during granulocytic differentiation of HL60 cells. / Chambers, Setsuko K; Gilmore-Hebert, Maureen; Kacinski, Barry M.; Benz, Edward J.

In: Blood, Vol. 80, No. 6, 15.09.1992, p. 1559-1564.

Research output: Contribution to journalArticle

Chambers, SK, Gilmore-Hebert, M, Kacinski, BM & Benz, EJ 1992, 'Changes in Na,K-ATPase gene expression during granulocytic differentiation of HL60 cells', Blood, vol. 80, no. 6, pp. 1559-1564.
Chambers SK, Gilmore-Hebert M, Kacinski BM, Benz EJ. Changes in Na,K-ATPase gene expression during granulocytic differentiation of HL60 cells. Blood. 1992 Sep 15;80(6):1559-1564.
Chambers, Setsuko K ; Gilmore-Hebert, Maureen ; Kacinski, Barry M. ; Benz, Edward J. / Changes in Na,K-ATPase gene expression during granulocytic differentiation of HL60 cells. In: Blood. 1992 ; Vol. 80, No. 6. pp. 1559-1564.
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