Changes in the crystallographic structures of cardiac myosin filaments detected by polarization-dependent second harmonic generation microscopy

C. A.I. Yuan, Zhonghai Wang, Thomas K. Borg, Tong Ye, Catalin Baicu, A. M.Y. Bradshaw, Michael Zile, Raymond B Runyan, Yonghong Shao, Bruce Z. Gao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Detecting the structural changes caused by volume and pressure overload is critical to comprehending the mechanisms of physiologic and pathologic hypertrophy. This study explores the structural changes at the crystallographic level in myosin filaments in volume-and pressure-overloaded myocardia through polarization-dependent second harmonic generation microscopy. Here, for the first time, we report that the ratio of nonlinear susceptibility tensor components d33/d15 increased significantly in volume-and pressure-overloaded myocardial tissues compared with the ratio in normal mouse myocardial tissues. Through cell stretch experiments, we demonstrated that mechanical tension plays an important role in the increase of d33/d15 in volume-and pressure-overloaded myocardial tissues.

Original languageEnglish (US)
Pages (from-to)3183-3195
Number of pages13
JournalBiomedical Optics Express
Volume10
Issue number7
DOIs
StatePublished - Jul 1 2019

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Cardiac Myosins
myosins
Microscopy
filaments
harmonic generations
microscopy
Pressure
polarization
myocardium
Myosins
Hypertrophy
mice
Myocardium
tensors
magnetic permeability
cells

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Changes in the crystallographic structures of cardiac myosin filaments detected by polarization-dependent second harmonic generation microscopy. / Yuan, C. A.I.; Wang, Zhonghai; Borg, Thomas K.; Ye, Tong; Baicu, Catalin; Bradshaw, A. M.Y.; Zile, Michael; Runyan, Raymond B; Shao, Yonghong; Gao, Bruce Z.

In: Biomedical Optics Express, Vol. 10, No. 7, 01.07.2019, p. 3183-3195.

Research output: Contribution to journalArticle

Yuan, C. A.I. ; Wang, Zhonghai ; Borg, Thomas K. ; Ye, Tong ; Baicu, Catalin ; Bradshaw, A. M.Y. ; Zile, Michael ; Runyan, Raymond B ; Shao, Yonghong ; Gao, Bruce Z. / Changes in the crystallographic structures of cardiac myosin filaments detected by polarization-dependent second harmonic generation microscopy. In: Biomedical Optics Express. 2019 ; Vol. 10, No. 7. pp. 3183-3195.
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