Changes in the expression of a2-adrenergic receptor subtypes during maturation of neuronal cells from fetal pig superior cervical ganglia

N. Fraeyman, P. Vanscheeuwijck, J. M. Wang, Y. Huang, W. P. De Potter, John W Regan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The expression of presynaptic a2-adrenergic receptor a2-AR subtypes was investigated in cultured neurons from fetal pig superior cervical ganglion SCG. Cells were incubated with chicken antibodies against a2A-, a2B- or a2C-AR subtypes either alone or together with antibodies against dopamine-b-hydroxylase DbH, a marker for adrenergic neurons or against choline acetyl transferase ChAT, a marker for cholinergic neurons. We found immunoreactivity for all three a2-AR subtypes in SCG-cells when cultured for 8-11 days. The relative expression of the a2A-subtype was approximately 1r3 of that of a2B- and a2C-AR. Co-localisation of all three a2-AR subtypes was observed in cells expressing DbH or ChAT. Increasing the potassium concentration in the culture medium increased the expression of DbH and decreased the expression of the a2A- and a2C-subtype without altering the expression of the a2B-subtype. Co-culture of neurons with pig splenocytes enhanced the expression of ChAT and decreased the expression of the a2B- subtype without altering the expression of a2A- and a2C-subtypes. Our results indicate that the three a2-receptor subtypes are expressed on both noradrenergic and cholinergic nerves. Induction of the noradrenergic phenotype favours the expression of the a2B-subtype over that of the a2A- and a2C-subtype. Conversely, enhancement of the cholinergic phenotype favours the expression of the a2A- and a2C-subtypes over that of the a2B- subtype. Our results suggest that the a2B-receptor is preferentially associated with noradrenergic nerve endings.

Original languageEnglish (US)
Pages (from-to)127-132
Number of pages6
JournalDevelopmental Brain Research
Volume116
Issue number2
DOIs
StatePublished - Sep 6 1999

Fingerprint

Superior Cervical Ganglion
Adrenergic Receptors
Cholinergic Agents
Swine
Phenotype
Neurons
Adrenergic Neurons
Cholinergic Neurons
Nerve Endings
Antibodies
Transferases
Coculture Techniques
Choline
Culture Media
Chickens
Cultured Cells
Potassium
dopamine beta hydroxylase deficiency

Keywords

  • a-Receptor subtype
  • Antibody
  • Immunostaining
  • In vitro cell culture
  • Presynaptic a-adrenoceptor
  • Superior cervical ganglion

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Changes in the expression of a2-adrenergic receptor subtypes during maturation of neuronal cells from fetal pig superior cervical ganglia. / Fraeyman, N.; Vanscheeuwijck, P.; Wang, J. M.; Huang, Y.; De Potter, W. P.; Regan, John W.

In: Developmental Brain Research, Vol. 116, No. 2, 06.09.1999, p. 127-132.

Research output: Contribution to journalArticle

Fraeyman, N. ; Vanscheeuwijck, P. ; Wang, J. M. ; Huang, Y. ; De Potter, W. P. ; Regan, John W. / Changes in the expression of a2-adrenergic receptor subtypes during maturation of neuronal cells from fetal pig superior cervical ganglia. In: Developmental Brain Research. 1999 ; Vol. 116, No. 2. pp. 127-132.
@article{28ebd9834db7489991feb51809ac84a5,
title = "Changes in the expression of a2-adrenergic receptor subtypes during maturation of neuronal cells from fetal pig superior cervical ganglia",
abstract = "The expression of presynaptic a2-adrenergic receptor a2-AR subtypes was investigated in cultured neurons from fetal pig superior cervical ganglion SCG. Cells were incubated with chicken antibodies against a2A-, a2B- or a2C-AR subtypes either alone or together with antibodies against dopamine-b-hydroxylase DbH, a marker for adrenergic neurons or against choline acetyl transferase ChAT, a marker for cholinergic neurons. We found immunoreactivity for all three a2-AR subtypes in SCG-cells when cultured for 8-11 days. The relative expression of the a2A-subtype was approximately 1r3 of that of a2B- and a2C-AR. Co-localisation of all three a2-AR subtypes was observed in cells expressing DbH or ChAT. Increasing the potassium concentration in the culture medium increased the expression of DbH and decreased the expression of the a2A- and a2C-subtype without altering the expression of the a2B-subtype. Co-culture of neurons with pig splenocytes enhanced the expression of ChAT and decreased the expression of the a2B- subtype without altering the expression of a2A- and a2C-subtypes. Our results indicate that the three a2-receptor subtypes are expressed on both noradrenergic and cholinergic nerves. Induction of the noradrenergic phenotype favours the expression of the a2B-subtype over that of the a2A- and a2C-subtype. Conversely, enhancement of the cholinergic phenotype favours the expression of the a2A- and a2C-subtypes over that of the a2B- subtype. Our results suggest that the a2B-receptor is preferentially associated with noradrenergic nerve endings.",
keywords = "a-Receptor subtype, Antibody, Immunostaining, In vitro cell culture, Presynaptic a-adrenoceptor, Superior cervical ganglion",
author = "N. Fraeyman and P. Vanscheeuwijck and Wang, {J. M.} and Y. Huang and {De Potter}, {W. P.} and Regan, {John W}",
year = "1999",
month = "9",
day = "6",
doi = "10.1016/S0165-3806(99)00078-4",
language = "English (US)",
volume = "116",
pages = "127--132",
journal = "Developmental Brain Research",
issn = "0165-3806",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Changes in the expression of a2-adrenergic receptor subtypes during maturation of neuronal cells from fetal pig superior cervical ganglia

AU - Fraeyman, N.

AU - Vanscheeuwijck, P.

AU - Wang, J. M.

AU - Huang, Y.

AU - De Potter, W. P.

AU - Regan, John W

PY - 1999/9/6

Y1 - 1999/9/6

N2 - The expression of presynaptic a2-adrenergic receptor a2-AR subtypes was investigated in cultured neurons from fetal pig superior cervical ganglion SCG. Cells were incubated with chicken antibodies against a2A-, a2B- or a2C-AR subtypes either alone or together with antibodies against dopamine-b-hydroxylase DbH, a marker for adrenergic neurons or against choline acetyl transferase ChAT, a marker for cholinergic neurons. We found immunoreactivity for all three a2-AR subtypes in SCG-cells when cultured for 8-11 days. The relative expression of the a2A-subtype was approximately 1r3 of that of a2B- and a2C-AR. Co-localisation of all three a2-AR subtypes was observed in cells expressing DbH or ChAT. Increasing the potassium concentration in the culture medium increased the expression of DbH and decreased the expression of the a2A- and a2C-subtype without altering the expression of the a2B-subtype. Co-culture of neurons with pig splenocytes enhanced the expression of ChAT and decreased the expression of the a2B- subtype without altering the expression of a2A- and a2C-subtypes. Our results indicate that the three a2-receptor subtypes are expressed on both noradrenergic and cholinergic nerves. Induction of the noradrenergic phenotype favours the expression of the a2B-subtype over that of the a2A- and a2C-subtype. Conversely, enhancement of the cholinergic phenotype favours the expression of the a2A- and a2C-subtypes over that of the a2B- subtype. Our results suggest that the a2B-receptor is preferentially associated with noradrenergic nerve endings.

AB - The expression of presynaptic a2-adrenergic receptor a2-AR subtypes was investigated in cultured neurons from fetal pig superior cervical ganglion SCG. Cells were incubated with chicken antibodies against a2A-, a2B- or a2C-AR subtypes either alone or together with antibodies against dopamine-b-hydroxylase DbH, a marker for adrenergic neurons or against choline acetyl transferase ChAT, a marker for cholinergic neurons. We found immunoreactivity for all three a2-AR subtypes in SCG-cells when cultured for 8-11 days. The relative expression of the a2A-subtype was approximately 1r3 of that of a2B- and a2C-AR. Co-localisation of all three a2-AR subtypes was observed in cells expressing DbH or ChAT. Increasing the potassium concentration in the culture medium increased the expression of DbH and decreased the expression of the a2A- and a2C-subtype without altering the expression of the a2B-subtype. Co-culture of neurons with pig splenocytes enhanced the expression of ChAT and decreased the expression of the a2B- subtype without altering the expression of a2A- and a2C-subtypes. Our results indicate that the three a2-receptor subtypes are expressed on both noradrenergic and cholinergic nerves. Induction of the noradrenergic phenotype favours the expression of the a2B-subtype over that of the a2A- and a2C-subtype. Conversely, enhancement of the cholinergic phenotype favours the expression of the a2A- and a2C-subtypes over that of the a2B- subtype. Our results suggest that the a2B-receptor is preferentially associated with noradrenergic nerve endings.

KW - a-Receptor subtype

KW - Antibody

KW - Immunostaining

KW - In vitro cell culture

KW - Presynaptic a-adrenoceptor

KW - Superior cervical ganglion

UR - http://www.scopus.com/inward/record.url?scp=0032853016&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032853016&partnerID=8YFLogxK

U2 - 10.1016/S0165-3806(99)00078-4

DO - 10.1016/S0165-3806(99)00078-4

M3 - Article

VL - 116

SP - 127

EP - 132

JO - Developmental Brain Research

JF - Developmental Brain Research

SN - 0165-3806

IS - 2

ER -