Changes in the expression of a2-adrenergic receptor subtypes during maturation of neuronal cells from fetal pig superior cervical ganglia

N. Fraeyman, P. Vanscheeuwijck, J. M. Wang, Y. Huang, W. P. De Potter, J. W. Regan

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The expression of presynaptic a2-adrenergic receptor a2-AR subtypes was investigated in cultured neurons from fetal pig superior cervical ganglion SCG. Cells were incubated with chicken antibodies against a2A-, a2B- or a2C-AR subtypes either alone or together with antibodies against dopamine-b-hydroxylase DbH, a marker for adrenergic neurons or against choline acetyl transferase ChAT, a marker for cholinergic neurons. We found immunoreactivity for all three a2-AR subtypes in SCG-cells when cultured for 8-11 days. The relative expression of the a2A-subtype was approximately 1r3 of that of a2B- and a2C-AR. Co-localisation of all three a2-AR subtypes was observed in cells expressing DbH or ChAT. Increasing the potassium concentration in the culture medium increased the expression of DbH and decreased the expression of the a2A- and a2C-subtype without altering the expression of the a2B-subtype. Co-culture of neurons with pig splenocytes enhanced the expression of ChAT and decreased the expression of the a2B- subtype without altering the expression of a2A- and a2C-subtypes. Our results indicate that the three a2-receptor subtypes are expressed on both noradrenergic and cholinergic nerves. Induction of the noradrenergic phenotype favours the expression of the a2B-subtype over that of the a2A- and a2C-subtype. Conversely, enhancement of the cholinergic phenotype favours the expression of the a2A- and a2C-subtypes over that of the a2B- subtype. Our results suggest that the a2B-receptor is preferentially associated with noradrenergic nerve endings.

Original languageEnglish (US)
Pages (from-to)127-132
Number of pages6
JournalDevelopmental Brain Research
Volume116
Issue number2
DOIs
StatePublished - Sep 6 1999

Keywords

  • Antibody
  • Immunostaining
  • In vitro cell culture
  • Presynaptic a-adrenoceptor
  • Superior cervical ganglion
  • a-Receptor subtype

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

Fingerprint Dive into the research topics of 'Changes in the expression of a<sub>2</sub>-adrenergic receptor subtypes during maturation of neuronal cells from fetal pig superior cervical ganglia'. Together they form a unique fingerprint.

Cite this