Chapter 6 Plasmon Resonance Methods in Membrane Protein Biology. Applications to GPCR Signaling

Zdzislaw Salamon, Gordon Tollin, Isabel Alves, Victor Hruby

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

Plasmon waveguide resonance (PWR) spectroscopy, a variant of surface plasmon resonance (SPR) spectrometry, allows one to examine changes in conformation of anisotropic structures such as membranes and membrane-associated proteins such as G-protein-coupled receptors (GPCRs). The binding and resulting structural changes that accompany interactions of membrane protein with ligands (agonists, antagonists, inverse agonist, etc.), G-proteins, and other effectors and modulators of signaling can be directly examined with this technique. In this chapter we outline the instrumentation used for these studies, the experimental methods that allow determination of the structural changes, and thermodynamic and kinetic parameters that can be obtained from these studies.

Original languageEnglish (US)
Title of host publicationChemokines
EditorsTracy Handel, Damon Hamel
Pages123-146
Number of pages24
EditionB
DOIs
StatePublished - May 27 2009

Publication series

NameMethods in Enzymology
NumberB
Volume461
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Salamon, Z., Tollin, G., Alves, I., & Hruby, V. (2009). Chapter 6 Plasmon Resonance Methods in Membrane Protein Biology. Applications to GPCR Signaling. In T. Handel, & D. Hamel (Eds.), Chemokines (B ed., pp. 123-146). (Methods in Enzymology; Vol. 461, No. B). https://doi.org/10.1016/S0076-6879(09)05406-8