Characterization and functional biology of the soybean aleurone layer

Research output: Contribution to journalArticle

Abstract

Background: Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean's genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments. Results: In this paper we describe the morphology and composition of the persistent single-cell aleurone layer that derives from the endosperm of developing soybean seeds. Its composition compared to cotyledonary tissue indicates the aleurone layer plays a role in both abiotic and biotic stress. The potential utility as the aleurone layer as a transient expression system in soybean was shown. As a near transparent single-cell layer it can be used as a transient expression system to study transgene expression and inter- and intra-cellular targeting as it is amenable to microscopic techniques. Conclusion: The transparent single cell aleurone layer was shown to be compositionally comparable to cotyledonary tissue in soybean with an enrichment in oxidative response proteins and shown to be a potential transient expression platform.

Original languageEnglish (US)
Article number354
JournalBMC Plant Biology
Volume18
Issue number1
DOIs
StatePublished - Dec 13 2018

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aleurone layer
soybeans
Biological Sciences
oilseed crops
cells
biotic stress
seeds
products and commodities
rapid methods
abiotic stress
endosperm
transgenes
genes
lipid content
protein content
genomics
genome
crops

Keywords

  • Aleurone
  • Seed-specific
  • Soybean
  • Sub-cellular localization
  • Transient expression

ASJC Scopus subject areas

  • Plant Science

Cite this

Characterization and functional biology of the soybean aleurone layer. / Schmidt, Monica; Herman, Eliot M.

In: BMC Plant Biology, Vol. 18, No. 1, 354, 13.12.2018.

Research output: Contribution to journalArticle

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