Abstract
Background: Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean's genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments. Results: In this paper we describe the morphology and composition of the persistent single-cell aleurone layer that derives from the endosperm of developing soybean seeds. Its composition compared to cotyledonary tissue indicates the aleurone layer plays a role in both abiotic and biotic stress. The potential utility as the aleurone layer as a transient expression system in soybean was shown. As a near transparent single-cell layer it can be used as a transient expression system to study transgene expression and inter- and intra-cellular targeting as it is amenable to microscopic techniques. Conclusion: The transparent single cell aleurone layer was shown to be compositionally comparable to cotyledonary tissue in soybean with an enrichment in oxidative response proteins and shown to be a potential transient expression platform.
| Original language | English (US) |
|---|---|
| Article number | 354 |
| Journal | BMC Plant Biology |
| Volume | 18 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 13 2018 |
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Keywords
- Aleurone
- Seed-specific
- Soybean
- Sub-cellular localization
- Transient expression
ASJC Scopus subject areas
- Plant Science
Cite this
Characterization and functional biology of the soybean aleurone layer. / Schmidt, Monica; Herman, Eliot M.
In: BMC Plant Biology, Vol. 18, No. 1, 354, 13.12.2018.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization and functional biology of the soybean aleurone layer
AU - Schmidt, Monica
AU - Herman, Eliot M
PY - 2018/12/13
Y1 - 2018/12/13
N2 - Background: Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean's genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments. Results: In this paper we describe the morphology and composition of the persistent single-cell aleurone layer that derives from the endosperm of developing soybean seeds. Its composition compared to cotyledonary tissue indicates the aleurone layer plays a role in both abiotic and biotic stress. The potential utility as the aleurone layer as a transient expression system in soybean was shown. As a near transparent single-cell layer it can be used as a transient expression system to study transgene expression and inter- and intra-cellular targeting as it is amenable to microscopic techniques. Conclusion: The transparent single cell aleurone layer was shown to be compositionally comparable to cotyledonary tissue in soybean with an enrichment in oxidative response proteins and shown to be a potential transient expression platform.
AB - Background: Soybean is a globally important oil seed crop. Both the high protein and oil content of soybean seeds make this crop a lucrative commodity. As in higher eukaryotic species with available genomes, the functional annotation of most of soybean's genes still remains to be investigated. A major hurdle in the functional genomics of soybean is a rapid method to test gene constructs before embarking on stable transformation experiments. Results: In this paper we describe the morphology and composition of the persistent single-cell aleurone layer that derives from the endosperm of developing soybean seeds. Its composition compared to cotyledonary tissue indicates the aleurone layer plays a role in both abiotic and biotic stress. The potential utility as the aleurone layer as a transient expression system in soybean was shown. As a near transparent single-cell layer it can be used as a transient expression system to study transgene expression and inter- and intra-cellular targeting as it is amenable to microscopic techniques. Conclusion: The transparent single cell aleurone layer was shown to be compositionally comparable to cotyledonary tissue in soybean with an enrichment in oxidative response proteins and shown to be a potential transient expression platform.
KW - Aleurone
KW - Seed-specific
KW - Soybean
KW - Sub-cellular localization
KW - Transient expression
UR - http://www.scopus.com/inward/record.url?scp=85058452632&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85058452632&partnerID=8YFLogxK
U2 - 10.1186/s12870-018-1579-8
DO - 10.1186/s12870-018-1579-8
M3 - Article
C2 - 30545296
AN - SCOPUS:85058452632
VL - 18
JO - BMC Plant Biology
JF - BMC Plant Biology
SN - 1471-2229
IS - 1
M1 - 354
ER -