Characterization and quantification of bacterial pathogens and indicator organisms in household kitchens with and without the use of a disinfectant cleaner

K. L. Josephson, J. R. Rubino, Ian L Pepper

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

This two year study evaluated the prevalence of indicator bacteria and specific pathogens in 10 'normal' kitchens in the United States. In Phase I, none of the kitchens was cleaned with an antimicrobial cleaner or disinfectant. Eight locations within the kitchens were monitored for: total heterotrophs, staphylococci, Pseudomonas, total coliforms and faecal coliforms. Almost all locations at all households exhibited contamination, with the sink and sponge samples exhibiting large bacterial concentrations. The faecal coliform concentrations in sink and sponge samples were very high, with 63 and 67% of all samples being positive, respectively. Escherichia coli was detected in 16·7% of all sink surfaces and 33·3% of all sponges. Salmonella was detected once and Campylobacter, on two occasions. In a second phase, households were provided with an antimicrobial disinfectant cleaner which families were encouraged to use but not forced to do so; in some cases, the product was used infrequently or not at all. This regimen did not demonstrate any consistent reduction in the incidence of bacterial contamination. By contrast, in the final phase of the study where disinfectant use was targeted for surfaces soon after contamination with foods or hands, the incidence of contamination decreased dramatically. These data show that normal kitchens can easily be contaminated with a variety of bacterial contaminants including faecal coliforms, E. coli, Salmonella and Campylobacter. Irregular use, or not using antimicrobial agents, is unlikely to reduce the risk of these infectious agents. By contrast, targeted use is likely to reduce the incidence of bacterial contaminants.

Original languageEnglish (US)
Pages (from-to)737-750
Number of pages14
JournalJournal of Applied Microbiology
Volume83
Issue number6
StatePublished - 1997

Fingerprint

kitchens
cleaners
Disinfectants
disinfectants
Porifera
indicator species
bacterial contamination
households
Campylobacter
Salmonella
pathogens
anti-infective agents
Incidence
incidence
Escherichia coli
Food Contamination
Anti-Infective Agents
Pseudomonas
Staphylococcus
heterotrophs

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

Cite this

@article{4f619e60a41a44798636c62695da1bf8,
title = "Characterization and quantification of bacterial pathogens and indicator organisms in household kitchens with and without the use of a disinfectant cleaner",
abstract = "This two year study evaluated the prevalence of indicator bacteria and specific pathogens in 10 'normal' kitchens in the United States. In Phase I, none of the kitchens was cleaned with an antimicrobial cleaner or disinfectant. Eight locations within the kitchens were monitored for: total heterotrophs, staphylococci, Pseudomonas, total coliforms and faecal coliforms. Almost all locations at all households exhibited contamination, with the sink and sponge samples exhibiting large bacterial concentrations. The faecal coliform concentrations in sink and sponge samples were very high, with 63 and 67{\%} of all samples being positive, respectively. Escherichia coli was detected in 16·7{\%} of all sink surfaces and 33·3{\%} of all sponges. Salmonella was detected once and Campylobacter, on two occasions. In a second phase, households were provided with an antimicrobial disinfectant cleaner which families were encouraged to use but not forced to do so; in some cases, the product was used infrequently or not at all. This regimen did not demonstrate any consistent reduction in the incidence of bacterial contamination. By contrast, in the final phase of the study where disinfectant use was targeted for surfaces soon after contamination with foods or hands, the incidence of contamination decreased dramatically. These data show that normal kitchens can easily be contaminated with a variety of bacterial contaminants including faecal coliforms, E. coli, Salmonella and Campylobacter. Irregular use, or not using antimicrobial agents, is unlikely to reduce the risk of these infectious agents. By contrast, targeted use is likely to reduce the incidence of bacterial contaminants.",
author = "Josephson, {K. L.} and Rubino, {J. R.} and Pepper, {Ian L}",
year = "1997",
language = "English (US)",
volume = "83",
pages = "737--750",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Characterization and quantification of bacterial pathogens and indicator organisms in household kitchens with and without the use of a disinfectant cleaner

AU - Josephson, K. L.

AU - Rubino, J. R.

AU - Pepper, Ian L

PY - 1997

Y1 - 1997

N2 - This two year study evaluated the prevalence of indicator bacteria and specific pathogens in 10 'normal' kitchens in the United States. In Phase I, none of the kitchens was cleaned with an antimicrobial cleaner or disinfectant. Eight locations within the kitchens were monitored for: total heterotrophs, staphylococci, Pseudomonas, total coliforms and faecal coliforms. Almost all locations at all households exhibited contamination, with the sink and sponge samples exhibiting large bacterial concentrations. The faecal coliform concentrations in sink and sponge samples were very high, with 63 and 67% of all samples being positive, respectively. Escherichia coli was detected in 16·7% of all sink surfaces and 33·3% of all sponges. Salmonella was detected once and Campylobacter, on two occasions. In a second phase, households were provided with an antimicrobial disinfectant cleaner which families were encouraged to use but not forced to do so; in some cases, the product was used infrequently or not at all. This regimen did not demonstrate any consistent reduction in the incidence of bacterial contamination. By contrast, in the final phase of the study where disinfectant use was targeted for surfaces soon after contamination with foods or hands, the incidence of contamination decreased dramatically. These data show that normal kitchens can easily be contaminated with a variety of bacterial contaminants including faecal coliforms, E. coli, Salmonella and Campylobacter. Irregular use, or not using antimicrobial agents, is unlikely to reduce the risk of these infectious agents. By contrast, targeted use is likely to reduce the incidence of bacterial contaminants.

AB - This two year study evaluated the prevalence of indicator bacteria and specific pathogens in 10 'normal' kitchens in the United States. In Phase I, none of the kitchens was cleaned with an antimicrobial cleaner or disinfectant. Eight locations within the kitchens were monitored for: total heterotrophs, staphylococci, Pseudomonas, total coliforms and faecal coliforms. Almost all locations at all households exhibited contamination, with the sink and sponge samples exhibiting large bacterial concentrations. The faecal coliform concentrations in sink and sponge samples were very high, with 63 and 67% of all samples being positive, respectively. Escherichia coli was detected in 16·7% of all sink surfaces and 33·3% of all sponges. Salmonella was detected once and Campylobacter, on two occasions. In a second phase, households were provided with an antimicrobial disinfectant cleaner which families were encouraged to use but not forced to do so; in some cases, the product was used infrequently or not at all. This regimen did not demonstrate any consistent reduction in the incidence of bacterial contamination. By contrast, in the final phase of the study where disinfectant use was targeted for surfaces soon after contamination with foods or hands, the incidence of contamination decreased dramatically. These data show that normal kitchens can easily be contaminated with a variety of bacterial contaminants including faecal coliforms, E. coli, Salmonella and Campylobacter. Irregular use, or not using antimicrobial agents, is unlikely to reduce the risk of these infectious agents. By contrast, targeted use is likely to reduce the incidence of bacterial contaminants.

UR - http://www.scopus.com/inward/record.url?scp=0031472294&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031472294&partnerID=8YFLogxK

M3 - Article

VL - 83

SP - 737

EP - 750

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 6

ER -