Characterization of a 34-kDa soybean binding protein for the syringolide elicitors

Cheng Ji, Carol Boyd, David Slaymaker, Yasushi Okinaka, Yoji Takeuchi, Sharon L. Midland, James J. Sims, Eliot M Herman, Noel Keen

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Syringolides are water-soluble, low-molecular-weight elicitors that trigger defense responses in soybean cultivars carrying the Rpg4 disease- resistance gene but not in rpg4 cultivars. 125I-syringolide 1 previously was shown to bind to a soluble protein(s) in extracts from soybean leaves. A 34-kDa protein that accounted for 125I-syringolide 1 binding activity was isolated with a syringolide affinity-gel column. Partial sequences of internal peptides of the 34-kDa protein were identical to P34, a previously described soybean seed allergen. In soybean seeds, P34 is processed from a 46-kDa precursor protein and was shown to have homology with thiol proteases. P34 is a moderately abundant protein in soybean seeds and cotyledons but its level in leaves is low. cDNAs encoding 46-, 34-, and 32-kDa forms of the soybean protein were cloned into the baculovirus vector, pVL1392, and expressed in insect cells. The resulting 32- and 34-kDa proteins, but not the 46-kDa protein, exhibited ligand-specific 125I-syringolide binding activity. These results suggest that P34 may be the receptor that mediates syringolide signaling.

Original languageEnglish (US)
Pages (from-to)3306-3311
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number6
DOIs
StatePublished - Mar 17 1998
Externally publishedYes

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Soybean Proteins
Carrier Proteins
Soybeans
Seeds
Proteins
Disease Resistance
Protein Precursors
Cotyledon
Baculoviridae
Sulfhydryl Compounds
Allergens
Insects
Peptide Hydrolases
Complementary DNA
Molecular Weight
Gels
Ligands
Peptides
Water
Genes

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Characterization of a 34-kDa soybean binding protein for the syringolide elicitors. / Ji, Cheng; Boyd, Carol; Slaymaker, David; Okinaka, Yasushi; Takeuchi, Yoji; Midland, Sharon L.; Sims, James J.; Herman, Eliot M; Keen, Noel.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 6, 17.03.1998, p. 3306-3311.

Research output: Contribution to journalArticle

Ji, Cheng ; Boyd, Carol ; Slaymaker, David ; Okinaka, Yasushi ; Takeuchi, Yoji ; Midland, Sharon L. ; Sims, James J. ; Herman, Eliot M ; Keen, Noel. / Characterization of a 34-kDa soybean binding protein for the syringolide elicitors. In: Proceedings of the National Academy of Sciences of the United States of America. 1998 ; Vol. 95, No. 6. pp. 3306-3311.
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AU - Sims, James J.

AU - Herman, Eliot M

AU - Keen, Noel

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