Characterization of a new strain of Taura syndrome virus (TSV) from Colombian shrimp farms and the implication in the selection of TSV resistant lines

Luis Fernando Aranguren, Marcela Salazar, Feng-Jyu Tang-Nelson, Xenia Caraballo, Donald V Lightner

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Prior to 2004, Colombian shrimp farming benefited from a selection program in which Penaeus vannamei stocks were developed with resistance to Taura syndrome disease (TS). However since 2004, TS reappeared as a significant disease. In 2010, an apparently new strain of TSV (designated as CO 10) was collected in Colombia. Its genome was sequenced and compared with six other fully sequenced isolates. This analysis revealed that the TSV CO 10 is closely related to the isolates from Hawaii and Venezuela. Phylogenetic analysis based on capsid protein 2 (CP2) region from 59 TSV isolates shows that the recent Colombian isolates (2006-2010) form a new cluster and differ from the previous Colombia isolates (1994-1998) by 4% in nucleotide sequence. The virulence of this CO 10 isolate was similar to a Belize TSV determined through experimental infection in P. vannamei showing 100% mortalities and similar survival curves. By RT-qPCR for TSV, the viral loads were also close in the infected shrimp from both CO 10 and Belize at the order of 1×1010copies per μl RNA. To develop TSV-resistant lines, the candidate shrimp should be challenged with virus strains that have been isolated most recently from the regions where they will be cultured. This study suggests that the TSV present in Colombian shrimp farms during the last 5years is a new TSV strain with high virulence.

Original languageEnglish (US)
Pages (from-to)68-73
Number of pages6
JournalJournal of Invertebrate Pathology
Volume112
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

Taura syndrome virus
virus
shrimp
farm
farms
Belize
Litopenaeus vannamei
Colombia
virulence
viral load
coat proteins
Hawaii
Venezuela
farming systems
RNA
genome
nucleotide sequences
viruses

Keywords

  • Dicistroviridae
  • Penaeus vannamei
  • Phylogenetic analysis
  • RT-qPCR
  • TSV
  • TSV-resistant lines

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

Cite this

Characterization of a new strain of Taura syndrome virus (TSV) from Colombian shrimp farms and the implication in the selection of TSV resistant lines. / Aranguren, Luis Fernando; Salazar, Marcela; Tang-Nelson, Feng-Jyu; Caraballo, Xenia; Lightner, Donald V.

In: Journal of Invertebrate Pathology, Vol. 112, No. 1, 01.2013, p. 68-73.

Research output: Contribution to journalArticle

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