Characterization of a nuclear factor that binds to AP1-like element in the rat p53 promoter during liver regeneration

Alexander D. Verin, Csilla Csortos, Steve D. Durbin, Antonina Aydanyan, Peiyi Wang, Carolyn E. Patterson, Joe GN Garcia

Research output: Contribution to journalArticle

Abstract

The transcription level of the rat p53 gene increases at 5-12 h in the regenerating liver after partial hepatectomy. It was previously reported that an activator protein 1 (AP1)-like element (-264 - 284) mediated the induced transcription of the rat p53 gene during liver regeneration. In this study, we characterize the protein binding to the AP1-like element by various methods. Oligonucleotide competition assays showed that the binding protein did not require AP1 consensus sequence. Therefore, the binding protein is not an AP1 family protein. Zn2+ was required for maximum DNA-binding activity of the protein, suggesting that the binding protein contains zinc fingers. The binding protein was highly resistant to denaturant. Even 1.8 M urea did not eliminate the protein-DNA complexes. In addition, the binding protein was stable up to 55°C. The protein-DNA complexes were abolished in the presence of 0.6 M NaCl and higher. Protease clipping assay showed that the protein had a protease-resistant core DNA binding domain. These results provided new insights into the structure of the protein that binds to the AP1-like element of the p53 promoter during liver regeneration. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish (US)
Pages (from-to)124-132
Number of pages9
JournalJournal of Cellular Biochemistry
Volume80
Issue number1
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • AP1-like element
  • Characterization
  • Liver regeneration
  • P53 promoter
  • Partial hepatectomy
  • Transcription regulation
  • Zinc finger

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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