Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase mRNA

Donna M. Voeller, Li Mind Changchien, Gladys F. Maley, Frank Maley, Teiji Takechi, Ross E. Turner, William "Bill" Montfort, Carmen J. Allegra, Edrard Chu

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Previous studies have shown that human TS mRNA translation is controlled by a negative autoregulatory mechanism. In this study, an RNA electrophoretic gel mobility shift assay confirmed a direct interaction between Escherichia coli (E.coll) TS protein and its own E.coli TS mRNA. Two cis-acting sequences in the E.coli TS mRNA protein-coding region were identified, with one site corresponding to nucleotides 207-460 and the second site corresponding to nucleotides 461-807. Each of these mRNA sequences bind TS with a relative affinity similar to that of the full-length E.coli TS mRNA sequence (IC50 = 1 nM). A third binding site was identified, corresponding to nucleotides 808-1015, although its relative affinity for TS (IC50 = 5.1 nM) was lower than that of the other two cis-acting elements. E.coli TS proteins with mutations in amino acids located within the nucleotide-binding region retained the ability to bind RNA while proteins with mutations at either the nucleotide active site cysteine (C146S) or at amino acids located within the folatebinding region were unable to bind TS mRNA. These studies suggest that the regions on E.coli TS defined by the folate-binding site and/or critical cysteine sulfhydryl groups may represent important RNA binding domains. Further evidence is presented which demonstrates that the direct interaction with TS results in in vitro repression of E.coliTS mRNA translation.

Original languageEnglish (US)
Pages (from-to)869-875
Number of pages7
JournalNucleic Acids Research
Volume23
Issue number5
StatePublished - Mar 11 1995

Fingerprint

Thymidylate Synthase
Messenger RNA
Escherichia coli
Escherichia Coli
Nucleotides
Interaction
Protein Biosynthesis
RNA
Proteins
Protein
Inhibitory Concentration 50
Cysteine
Binding sites
Binding Sites
Amino Acids
Affine transformation
Amino acids
Mutation
Escherichia coli Proteins
Electrophoretic Mobility Shift Assay

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Voeller, D. M., Changchien, L. M., Maley, G. F., Maley, F., Takechi, T., Turner, R. E., ... Chu, E. (1995). Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase mRNA. Nucleic Acids Research, 23(5), 869-875.

Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase mRNA. / Voeller, Donna M.; Changchien, Li Mind; Maley, Gladys F.; Maley, Frank; Takechi, Teiji; Turner, Ross E.; Montfort, William "Bill"; Allegra, Carmen J.; Chu, Edrard.

In: Nucleic Acids Research, Vol. 23, No. 5, 11.03.1995, p. 869-875.

Research output: Contribution to journalArticle

Voeller, DM, Changchien, LM, Maley, GF, Maley, F, Takechi, T, Turner, RE, Montfort, WB, Allegra, CJ & Chu, E 1995, 'Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase mRNA', Nucleic Acids Research, vol. 23, no. 5, pp. 869-875.
Voeller, Donna M. ; Changchien, Li Mind ; Maley, Gladys F. ; Maley, Frank ; Takechi, Teiji ; Turner, Ross E. ; Montfort, William "Bill" ; Allegra, Carmen J. ; Chu, Edrard. / Characterization of a specific interaction between Escherichia coli thymidylate synthase and Escherichia coli thymidylate synthase mRNA. In: Nucleic Acids Research. 1995 ; Vol. 23, No. 5. pp. 869-875.
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