Characterization of a [3H]glutamate binding site in rat pineal gland: Enhanced affinity following superior cervical ganglionectomy

Laura Kus, Robert J. Handa, John A. McNulty

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Abstract: Glutamate, an excitatory neurotransmitter/neuromodulator involved in cell‐to‐cell communication within the central nervous system, is now believed to play a role in neuroendocrine function. In this study we describe a single, saturable, stereospecific, and temperature‐, time‐, and pH‐dependent binding site for glutamate in the pineal gland of the rat (Kd= 612 ± 23 nM, Bmax= 3.17 ± 0.33 pmol/mg protein). After removal of the sympathetic innervation to the pineal gland, [3H]glutamate binding displayed a higher apparent affinity (Kd= 412 ± 28 nM) (P < 0.05) without a change in binding site number (Bmax= 3.60 ± 0.24 pmol/mg protein). No difference in [3H]glutamate binding site number was observed in pineal glands obtained from animals sacrificed during the middle of the light and dark periods. These data suggest a possible modulatory role for a glutamate binding site in pineal gland function.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalJournal of Pineal Research
Volume14
Issue number1
DOIs
StatePublished - Jan 1993
Externally publishedYes

Keywords

  • autoradiography—
  • excitatory amino acid—
  • glutamate—
  • pineal gland—
  • superior cervical sympathectomy

ASJC Scopus subject areas

  • Endocrinology

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