Characterization of a [3H]glutamate binding site in rat pineal gland: Enhanced affinity following superior cervical ganglionectomy

L. Kus, Robert J Handa, J. A. McNulty

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Glutamate, an excitatory neurotransmitter/neuromodulator involved in cell-to-cell communication within the central nervous system, is now believed to play a role in neuroendocrine function. In this study we describe a single, saturable, stereospecific, and temperature-, time-, and pH-dependent binding site for glutamate in the pineal gland of the rat (K(d) = 612 ± 23 nM, B(max) = 3.17 ± 0.33 pmol/mg protein). After removal of the sympathetic innervation to the pineal gland, [3H]glutamate binding displayed a higher apparent affinity (K(d) = 412 ± 28 nM) (P < 0.05) without a change in binding site number (B(max) = 3.60 ± 0.24 pmol/mg protein). No difference in [3H]glutamate binding site number was observed in pineal glands obtained from animals sacrificed during the middle of the light and dark periods. These data suggest a possible modulatory role for a glutamate binding site inpineal gland function.

Original languageEnglish (US)
Pages (from-to)39-44
Number of pages6
JournalJournal of Pineal Research
Volume14
Issue number1
StatePublished - 1993
Externally publishedYes

Fingerprint

Ganglionectomy
Pineal Gland
Glutamic Acid
Binding Sites
Neurotransmitter Agents
Cell Communication
Proteins
Central Nervous System
Light
Temperature

Keywords

  • autoradiography
  • excitatory amino acid
  • glutamate
  • pineal gland
  • superior cervical sympathectomy

ASJC Scopus subject areas

  • Endocrinology

Cite this

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title = "Characterization of a [3H]glutamate binding site in rat pineal gland: Enhanced affinity following superior cervical ganglionectomy",
abstract = "Glutamate, an excitatory neurotransmitter/neuromodulator involved in cell-to-cell communication within the central nervous system, is now believed to play a role in neuroendocrine function. In this study we describe a single, saturable, stereospecific, and temperature-, time-, and pH-dependent binding site for glutamate in the pineal gland of the rat (K(d) = 612 ± 23 nM, B(max) = 3.17 ± 0.33 pmol/mg protein). After removal of the sympathetic innervation to the pineal gland, [3H]glutamate binding displayed a higher apparent affinity (K(d) = 412 ± 28 nM) (P < 0.05) without a change in binding site number (B(max) = 3.60 ± 0.24 pmol/mg protein). No difference in [3H]glutamate binding site number was observed in pineal glands obtained from animals sacrificed during the middle of the light and dark periods. These data suggest a possible modulatory role for a glutamate binding site inpineal gland function.",
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author = "L. Kus and Handa, {Robert J} and McNulty, {J. A.}",
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T1 - Characterization of a [3H]glutamate binding site in rat pineal gland

T2 - Enhanced affinity following superior cervical ganglionectomy

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AU - Handa, Robert J

AU - McNulty, J. A.

PY - 1993

Y1 - 1993

N2 - Glutamate, an excitatory neurotransmitter/neuromodulator involved in cell-to-cell communication within the central nervous system, is now believed to play a role in neuroendocrine function. In this study we describe a single, saturable, stereospecific, and temperature-, time-, and pH-dependent binding site for glutamate in the pineal gland of the rat (K(d) = 612 ± 23 nM, B(max) = 3.17 ± 0.33 pmol/mg protein). After removal of the sympathetic innervation to the pineal gland, [3H]glutamate binding displayed a higher apparent affinity (K(d) = 412 ± 28 nM) (P < 0.05) without a change in binding site number (B(max) = 3.60 ± 0.24 pmol/mg protein). No difference in [3H]glutamate binding site number was observed in pineal glands obtained from animals sacrificed during the middle of the light and dark periods. These data suggest a possible modulatory role for a glutamate binding site inpineal gland function.

AB - Glutamate, an excitatory neurotransmitter/neuromodulator involved in cell-to-cell communication within the central nervous system, is now believed to play a role in neuroendocrine function. In this study we describe a single, saturable, stereospecific, and temperature-, time-, and pH-dependent binding site for glutamate in the pineal gland of the rat (K(d) = 612 ± 23 nM, B(max) = 3.17 ± 0.33 pmol/mg protein). After removal of the sympathetic innervation to the pineal gland, [3H]glutamate binding displayed a higher apparent affinity (K(d) = 412 ± 28 nM) (P < 0.05) without a change in binding site number (B(max) = 3.60 ± 0.24 pmol/mg protein). No difference in [3H]glutamate binding site number was observed in pineal glands obtained from animals sacrificed during the middle of the light and dark periods. These data suggest a possible modulatory role for a glutamate binding site inpineal gland function.

KW - autoradiography

KW - excitatory amino acid

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KW - superior cervical sympathectomy

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