Characterization of an Escherichia coli plasmid encoding for synthesis of heat-labile toxin: molecular cloning of the toxin determinant

M. So, W. S. Dallas, S. Falkow

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

P307 is a plasmid isolated from a strain of E. coli that was responsible for an outbreak of diarrheal disease in piglets. This 60x106-dalton plasmid was subsequently shown to encode for the synthesis of a heat-labile toxin (LT). Using recombinant DNA technology the authors isolated a 7.8x106-dalton DNA fragment that contains the LT gene(s). This fragment was generated using an EcoRI partial digestion of P307 DNA, and the fragment was joined to a small multicopy plasmid, RSF2124. E. coli strains harboring the chimeric plasmid produced greater amounts of LT than did the same strains containing P307. The LT genes were also isolated on a 5.8x106-dalton DNA fragment made by BamHI digestion, and we identified an EcoRI recognition sequence that is located in a postion essential for LT synthesis.

Original languageEnglish (US)
Pages (from-to)405-411
Number of pages7
JournalInfection and Immunity
Volume21
Issue number2
DOIs
StatePublished - 1978
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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