Characterization of anopheles gambiae (African malaria mosquito) ferritin and the effect of iron on intracellular localization in mosquito cells

Dawn L. Geiser, Zachary R. Conley, Jamie L. Elliott, Jonathan J. Mayo, Joy Winzerling

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ferritin is a 24-subunit molecule, made up of heavy chain (HC) and light chain (LC) subunits, which stores and controls the release of dietary iron in mammals, plants, and insects. In mosquitoes, dietary iron taken in a bloodmeal is stored inside ferritin. Our previous work has demonstrated the transport of dietary iron to the ovaries via ferritin during oogenesis. We evaluated the localization of ferritin subunits inside CCL-125 [Aedes aegypti Linnaeus (Diptera: Culicidae), yellow fever mosquito] and 4a3b [Anopheles gambiae Giles (Diptera: Culicidae), African malaria mosquito] cells under various iron treatment conditions to further elucidate the regulation of iron metabolism in these important disease vectors and to observe the dynamics of the intracellular ferritin subunits following iron administration. Deconvolution microscopy captured 3D fluorescent images of iron-treated mosquito cells to visualize the ferritin HC and LC homologue subunits (HCH and LCH, respectively) in multiple focal planes. Fluorescent probes were used to illuminate cell organ-elles (i.e., Golgi apparatus, lysosomes, and nuclei) while secondary probes for specific ferritin subunits demonstrated abundance and co-localization within organelles. These images will help to develop a model for the biochemical regulation of ferritin under conditions of iron exposure, and to advance novel hypotheses for the crucial role of iron in mosquito vectors.

Original languageEnglish (US)
JournalJournal of Insect Science
Volume15
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Anopheles gambiae
ferritin
malaria
Culicidae
iron
cells
Aedes aegypti
disease vectors
lysosomes
oogenesis
Golgi apparatus
organelles
microscopy
mammals
insects
metabolism

Keywords

  • 4a3b
  • Aedes aegypti
  • CCL-125
  • Deconvolution microscopy

ASJC Scopus subject areas

  • Insect Science

Cite this

Characterization of anopheles gambiae (African malaria mosquito) ferritin and the effect of iron on intracellular localization in mosquito cells. / Geiser, Dawn L.; Conley, Zachary R.; Elliott, Jamie L.; Mayo, Jonathan J.; Winzerling, Joy.

In: Journal of Insect Science, Vol. 15, No. 1, 01.01.2015.

Research output: Contribution to journalArticle

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