Characterization of Arabidopsis AtUGT85A and AtGUS gene families and their expression in rapidly dividing tissues

Ho Hyung Woo, Byeong Ryong Jeong, Ann M. Hirsch, Martha C Hawes

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In humans, uridine 5′-diphosphate glucuronosyltransferase (UGT) operates in opposition to glucuronidase (GUS) to control activity of diverse metabolites such as hormones by reversible conjugation with glucuronic acid. Previous data revealed that, as in mammals, these enzymes are required for plant life in that a UGT from Pisum sativum (PsUGT1) controls plant development by opposing endogenous GUS activity thereby modulating the duration of the cell cycle. Here we report that a small family of genes (AtUGT85A1, 2, 3, 4, 5, and 7) homologous to pea PsUGT1 exists in the Arabidopsis genome. The AtUGT85A-encoded proteins are predicted to be membrane-associated enzymes. Three genes (AtGUS1, AtGUS2, and AtGUS3) that are homologous to a GUS-encoding gene from Scutellaria baicalensis were identified. The AtGUS-encoded proteins are predicted to be secretory (AtGUS1) and membrane-associated (AtGUS2 and AtGUS3) enzymes. Both AtUGT85A and AtGUS genes, like PsUGT1, exhibit localized, tissue-specific expression, mainly in areas of active cell division with possible involvement in cell cycle regulation.

Original languageEnglish (US)
Pages (from-to)143-153
Number of pages11
JournalGenomics
Volume90
Issue number1
DOIs
StatePublished - Jul 2007

Fingerprint

Arabidopsis
Glucuronidase
Glucuronosyltransferase
Uridine Diphosphate
Peas
Genes
Cell Cycle
Enzymes
Scutellaria baicalensis
Glucuronic Acid
Plant Development
Membranes
Cell Division
Mammals
Proteins
Genome
Hormones

Keywords

  • Arabidopsis AtUGT85A family
  • Glucuronidase
  • Lethality
  • UDP-glycosyltransferase

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of Arabidopsis AtUGT85A and AtGUS gene families and their expression in rapidly dividing tissues. / Woo, Ho Hyung; Jeong, Byeong Ryong; Hirsch, Ann M.; Hawes, Martha C.

In: Genomics, Vol. 90, No. 1, 07.2007, p. 143-153.

Research output: Contribution to journalArticle

Woo, Ho Hyung ; Jeong, Byeong Ryong ; Hirsch, Ann M. ; Hawes, Martha C. / Characterization of Arabidopsis AtUGT85A and AtGUS gene families and their expression in rapidly dividing tissues. In: Genomics. 2007 ; Vol. 90, No. 1. pp. 143-153.
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