Characterization of Imcrop, a Mutator-like MITE family in the rice genome

Young Jun Mo, Ki Young Kim, Woon Chul Shin, Gun Mi Lee, Jong Cheol Ko, Jeong Kwon Nam, Bo Kyeong Kim, Jae Kwon Ko, Yeisoo Yu, Tae Jin Yang

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Sequence comparisons of ammonium transporter 1-2 genes (OsAMT1-2) in different rice accessions revealed a MITE insertion in the upstream region of the gene. The 391-bp MITE, classified as a Mutator superfamily member and named Imcrop, included terminal inverted repeat (TIR) and 9-bp target site duplication (TSD) sequences. We identified 151 Imcrop elements dispersed on 12 chromosomes of the japonica reference genome. Of these, 12.6% were found in genic regions and 33.1% were located within 1.5 kb of annotated rice genes. We constructed comparative insertion maps with 111 and 102 intact Imcrop elements in the japonica and indica reference genomes, respectively. The Imcrop elements showed relatively even distribution across all chromosomes although their frequency was higher on chromosomes 1, 3, and 4 in both genomes. Seventy seven Imcrop elements were detected in both subspecies, whereas 34 and 25 insertions were found only in the japonica or indica genome, respectively. We compared insertion polymorphisms of 19 Imcrop elements found inside genes in 48 Korean rice cultivars, consisting of 42 japonica and six Tongil-types (indica-japonica cross). Thirteen insertions were common to all cultivars indicating these elements were present before indica-japonica divergence. The six other elements showed insertion polymorphisms among accessions, showing their recent insertion history or no critical positive effect of their insertion on the rice genome.

Original languageEnglish (US)
Pages (from-to)189-198
Number of pages10
JournalGenes and Genomics
Volume34
Issue number2
DOIs
StatePublished - Apr 1 2012

Keywords

  • Imcrop
  • Insertion polymorphism
  • Miniature inverted-repeat transposable element (MITE)
  • Rice

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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    Mo, Y. J., Kim, K. Y., Shin, W. C., Lee, G. M., Ko, J. C., Nam, J. K., Kim, B. K., Ko, J. K., Yu, Y., & Yang, T. J. (2012). Characterization of Imcrop, a Mutator-like MITE family in the rice genome. Genes and Genomics, 34(2), 189-198. https://doi.org/10.1007/s13258-011-0193-z