Characterization of phosphatase and tensin homolog expression in the mosquito Aedes aegypti

Six splice variants with developmental and tissue specificity

Michael A Riehle, Jessica M. Brown

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Phosphatase and tensin homologue (PTEN), an inhibitor of insulin signalling, was characterized in Aedes aegypti. Surprisingly, six splice variants were identified: three with alternative terminal exons (AaegPTEN2:3:6) and three formed by intron retention (AaegPTEN1:4:5). All variants encoded active phosphatase domains. Variants with alternative terminal exons also encoded C2 and COOH-domains, and AaegPTEN6 encoded a PDZ binding motif. These three variants also had unique expression patterns. AaegPTEN2 was expressed primarily in the ovary. AaegPTEN3 was predominant in heads and midguts, and throughout development, except early embryogenesis. AaegPTEN6 was expressed in fat body, ovaries, and throughout development. Intron retention variants were weakly expressed in most samples. These expression patterns suggest that AaegPTEN variants play unique roles in regulating insulin's pleiotropic effects.

Original languageEnglish (US)
Pages (from-to)277-286
Number of pages10
JournalInsect Molecular Biology
Volume16
Issue number3
DOIs
StatePublished - Jun 2007

Fingerprint

Organ Specificity
Aedes
Aedes aegypti
Culicidae
Phosphoric Monoester Hydrolases
Introns
exons
introns
Ovary
Exons
insulin
Tissue
Fat Body
Insulin
fat body
midgut
Embryonic Development
early development
embryogenesis
Fats

Keywords

  • Aedes aegypti
  • Insulin signalling
  • MMAC1
  • Phosphatidylinositol 3-kinase
  • PI(3,4,5)P3
  • PTEN
  • TEP1

ASJC Scopus subject areas

  • Insect Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

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title = "Characterization of phosphatase and tensin homolog expression in the mosquito Aedes aegypti: Six splice variants with developmental and tissue specificity",
abstract = "Phosphatase and tensin homologue (PTEN), an inhibitor of insulin signalling, was characterized in Aedes aegypti. Surprisingly, six splice variants were identified: three with alternative terminal exons (AaegPTEN2:3:6) and three formed by intron retention (AaegPTEN1:4:5). All variants encoded active phosphatase domains. Variants with alternative terminal exons also encoded C2 and COOH-domains, and AaegPTEN6 encoded a PDZ binding motif. These three variants also had unique expression patterns. AaegPTEN2 was expressed primarily in the ovary. AaegPTEN3 was predominant in heads and midguts, and throughout development, except early embryogenesis. AaegPTEN6 was expressed in fat body, ovaries, and throughout development. Intron retention variants were weakly expressed in most samples. These expression patterns suggest that AaegPTEN variants play unique roles in regulating insulin's pleiotropic effects.",
keywords = "Aedes aegypti, Insulin signalling, MMAC1, Phosphatidylinositol 3-kinase, PI(3,4,5)P3, PTEN, TEP1",
author = "Riehle, {Michael A} and Brown, {Jessica M.}",
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AU - Brown, Jessica M.

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N2 - Phosphatase and tensin homologue (PTEN), an inhibitor of insulin signalling, was characterized in Aedes aegypti. Surprisingly, six splice variants were identified: three with alternative terminal exons (AaegPTEN2:3:6) and three formed by intron retention (AaegPTEN1:4:5). All variants encoded active phosphatase domains. Variants with alternative terminal exons also encoded C2 and COOH-domains, and AaegPTEN6 encoded a PDZ binding motif. These three variants also had unique expression patterns. AaegPTEN2 was expressed primarily in the ovary. AaegPTEN3 was predominant in heads and midguts, and throughout development, except early embryogenesis. AaegPTEN6 was expressed in fat body, ovaries, and throughout development. Intron retention variants were weakly expressed in most samples. These expression patterns suggest that AaegPTEN variants play unique roles in regulating insulin's pleiotropic effects.

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