Characterization of protein I from serum-sensitive and serum-resistant transformants of Neisseria gonorrhoeae

R. C. Judd, M. Tam, Keith A Joiner

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The protein IAs of serum-sensitive (FA635) and serum-resistant (FA638) transformants of Neisseria gonorrhoeae, which have identical pedigrees, have been shown to be different by the use of a monoclonal antibody and were also shown to be different by proteinase K cleavage and primary structural and surface peptide mapping. The difference in structure is within the surface-exposed region of the molecule. The only other difference observed between the two strains was a very slight difference in lipooligosaccharide silver staining in sodium dodecyl sulfate-polyacrylamide gel electrophoresis. These data suggest that protein I alone or in combination with lipooligosaccharide may significantly contribute to serum resistance.

Original languageEnglish (US)
Pages (from-to)273-276
Number of pages4
JournalInfection and Immunity
Volume55
Issue number1
StatePublished - 1987
Externally publishedYes

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Neisseria gonorrhoeae
Blood Proteins
Endopeptidase K
Silver Staining
Peptide Mapping
Pedigree
Serum
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Monoclonal Antibodies
Proteins
lipid-linked oligosaccharides

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of protein I from serum-sensitive and serum-resistant transformants of Neisseria gonorrhoeae. / Judd, R. C.; Tam, M.; Joiner, Keith A.

In: Infection and Immunity, Vol. 55, No. 1, 1987, p. 273-276.

Research output: Contribution to journalArticle

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