Characterization of responding cells in oxidative mitogen stimulation. I. Ia + cells and Ly-1 +2 + cells are required for the proliferative response

M. L. Phillips, P. J. Wettstein, R. L. O'Brien, J. W. Parker, J. A. Frelinger

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Addition of anti-Ia sera to cultures of mouse spleen cells stimulated with oxidative mitogens, neuraminidase/galactose oxidase (NaGO) or sodium periodate (NaIO 4), inhibits the subsequent proliferative response 30 to 70%. Anti-H-2K or D sera were not specifically inhibitory. Similar inhibition was seen when cells were pretreated with anti-Ia sera and washed before exposure to the mitogenic enzymes. Treatment with anti-Ia serum and complement depletes greater than 89% of the NaGO and the NaIO 4 responses but 2% or less of the phytohemagglutinin (PHA) response. The response to NaGO was sensitive to depletion with anti-Thy-1 serum, rabbit antimouse brain serum, anti-Ly-1, or anti-Ly-2 serum. Mixtures of Ly-1 and Ly-2-depleted populations did not restore responsiveness. Thus both an Ia + cell and an Ly-1 +2 + T cell are required for [ 3H]TdR incorporation in response to NaGO treatment.

Original languageEnglish (US)
Pages (from-to)2693-2699
Number of pages7
JournalJournal of Immunology
Volume124
Issue number6
StatePublished - Jul 18 1980
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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