Characterization of somatic embryogenesis and plant regeneration in cotton (Gossypium hirsutum L.)

R. C. Shoemaker, L. J. Couche, D. W. Galbraith

Research output: Contribution to journalArticle

81 Scopus citations

Abstract

Seventeen cultivars of cotton (Gossypium hirsutum L.) were evaluated for callus initiation and maintenance using 3 initiation media and 3 maintenance media. After a series of transfers of a 3% glucose media, calli were placed on a 3% sucrose medium. After several weeks calli were observed for the presence of embryo-like structures. Cultivars Coker 201 and Coker 315 were identified as embryogenic. Embryogenic callus has since been routinely obtained within 6 weeks by initiating callus on glucose media for 3-4 weeks followed by transfer to sucrose media. Histological examination has shown that embryos are derived from isodiametric, densely cytoplasmic cells and follow predictable patterns of development. Upon maturity, transfer to auxin-free media with reduced sucrose levels results in embryo germination. Regenerated plants can be transferred to greenhouse within 90 days of callus initiation.

Original languageEnglish (US)
Pages (from-to)178-181
Number of pages4
JournalPlant Cell Reports
Volume5
Issue number3
DOIs
StatePublished - Jun 1 1986
Externally publishedYes

Keywords

  • Genotype
  • Gossypium hirsutum
  • Morphogenesis
  • Regeneration
  • Somatic embryos

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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