Abstract
The Bordetella pertussis gene sodB, encoding Superoxide dismutase (SOD), was cloned by complementation of an Escherichia coli sodAsodB double mutant. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to manganese- and iron-containing SODs from other organisms. Examination of SOD activity on gels suggests that B. pertussis extracts have a single SOD containing Fe+ as a prosthetic group. A SOD-deβcient mutant was obtained by insertional inactivation of sodB in B. pertussis, confirming that there is only one SOD in this organism.
Original language | English (US) |
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Pages (from-to) | 85-89 |
Number of pages | 5 |
Journal | Gene |
Volume | 142 |
Issue number | 1 |
DOIs | |
State | Published - May 3 1994 |
Keywords
- Oxidative stress
- homologous recombination
- human pathogen
- insertion mutagenesis
- metalloenzyme
- suicide vector
ASJC Scopus subject areas
- Genetics