Abstract
The Bordetella pertussis gene sodB, encoding Superoxide dismutase (SOD), was cloned by complementation of an Escherichia coli sodAsodB double mutant. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to manganese- and iron-containing SODs from other organisms. Examination of SOD activity on gels suggests that B. pertussis extracts have a single SOD containing Fe+ as a prosthetic group. A SOD-deβcient mutant was obtained by insertional inactivation of sodB in B. pertussis, confirming that there is only one SOD in this organism.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 85-89 |
| Number of pages | 5 |
| Journal | Gene |
| Volume | 142 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 3 1994 |
Keywords
- Oxidative stress
- homologous recombination
- human pathogen
- insertion mutagenesis
- metalloenzyme
- suicide vector
ASJC Scopus subject areas
- Genetics
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Cite this
DeShazer, D., Barnnan, J. D., Moran, M. J., & Friedman, R. L. (1994). Characterization of the gene encoding Superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant. Gene, 142(1), 85-89. https://doi.org/10.1016/0378-1119(94)90359-X