Characterization of the gene encoding Superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant

David DeShazer, Jason D. Barnnan, Michael J. Moran, Richard L Friedman

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The Bordetella pertussis gene sodB, encoding Superoxide dismutase (SOD), was cloned by complementation of an Escherichia coli sodAsodB double mutant. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to manganese- and iron-containing SODs from other organisms. Examination of SOD activity on gels suggests that B. pertussis extracts have a single SOD containing Fe+ as a prosthetic group. A SOD-deβcient mutant was obtained by insertional inactivation of sodB in B. pertussis, confirming that there is only one SOD in this organism.

Original languageEnglish (US)
Pages (from-to)85-89
Number of pages5
JournalGene
Volume142
Issue number1
DOIs
StatePublished - May 3 1994

Fingerprint

Bordetella pertussis
Superoxide Dismutase
Genes
Manganese
Iron
Gels
Escherichia coli
Proteins

Keywords

  • homologous recombination
  • human pathogen
  • insertion mutagenesis
  • metalloenzyme
  • Oxidative stress
  • suicide vector

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of the gene encoding Superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant. / DeShazer, David; Barnnan, Jason D.; Moran, Michael J.; Friedman, Richard L.

In: Gene, Vol. 142, No. 1, 03.05.1994, p. 85-89.

Research output: Contribution to journalArticle

DeShazer, David ; Barnnan, Jason D. ; Moran, Michael J. ; Friedman, Richard L. / Characterization of the gene encoding Superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant. In: Gene. 1994 ; Vol. 142, No. 1. pp. 85-89.
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