Characterization of the murine Icam-1 gene

Christie M. Ballantyne, James E Sligh, Xiao Yuan Dai, Arthur L. Beaudet

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Intercellular adhesion molecule-1 (ICAM-1, CD54) is a cell adhesion molecule that interacts with the leukocyte β2 integrins, LFA-1 and Mac-1. Murine inflammatory models are being utilized increasingly to define the role that ICAM-1 induction plays in the initiation of inflammation. We have isolated murine genomic clones that contain the Icam-1 gene including over 2 kb of 5′ flanking sequence. The gene for murine Icam-1 spans over 13 kb and is composed of seven exons and six introns. Each of the extracellular immunoglobulin-like domains of ICAM-1 is encoded by a single exon that ends with the first base of the next codon. Examination of ICAM-1 expression in vivo shows that mRNA levels of ICAM-1 are low in all organs except for the lung but increase markedly in multiple organs at 3 h after administration of endotoxin. The 5′ flanking region of the murine gene contains a putative TATA box and potential SP-1, AP-1, and AP-3 sites in positions nearly identical to those in the human gene.

Original languageEnglish (US)
Pages (from-to)1076-1080
Number of pages5
JournalGenomics
Volume14
Issue number4
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Intercellular Adhesion Molecule-1
Genes
5' Flanking Region
Exons
Lymphocyte Function-Associated Antigen-1
TATA Box
Transcription Factor AP-1
Cell Adhesion Molecules
Endotoxins
Integrins
Codon
Introns
Leukocytes
Clone Cells
Inflammation
Lung
Messenger RNA

ASJC Scopus subject areas

  • Genetics

Cite this

Ballantyne, C. M., Sligh, J. E., Dai, X. Y., & Beaudet, A. L. (1992). Characterization of the murine Icam-1 gene. Genomics, 14(4), 1076-1080. https://doi.org/10.1016/S0888-7543(05)80132-6

Characterization of the murine Icam-1 gene. / Ballantyne, Christie M.; Sligh, James E; Dai, Xiao Yuan; Beaudet, Arthur L.

In: Genomics, Vol. 14, No. 4, 1992, p. 1076-1080.

Research output: Contribution to journalArticle

Ballantyne, CM, Sligh, JE, Dai, XY & Beaudet, AL 1992, 'Characterization of the murine Icam-1 gene', Genomics, vol. 14, no. 4, pp. 1076-1080. https://doi.org/10.1016/S0888-7543(05)80132-6
Ballantyne, Christie M. ; Sligh, James E ; Dai, Xiao Yuan ; Beaudet, Arthur L. / Characterization of the murine Icam-1 gene. In: Genomics. 1992 ; Vol. 14, No. 4. pp. 1076-1080.
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