Characterization of the rat intestinal Fc receptor (FcRn) promoter: Transcriptional regulation of FcRn gene by the Sp family of transcription factors

Lingling Jiang, Jiafang Wang, R. Sergio Solorzano-Vargas, Hugh V. Tsai, Edgar M. Gutierrez, Luis O. Ontiveros, Pawel R Kiela, S. Vincent Wu, Martín G. Martín

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The regulatory elements that control the transcriptional regulation of the intestinal Fc receptor (FcRn) have not been elucidated. The objective of this study was to characterize the core promoter region of the rat FcRn gene. Chimeric clones that contained various regions of the promoter located upstream of the luciferase reporter were transiently transfected into either IEC-6 or Caco-2 cell lines and nuclear extracts were used to perform DNase I footprint and DNA binding assays (EMSA). Transfection of chimeric upstream nested deletions-luciferase reporter clones into either of these cell lines supported robust reporter activity and identified the location of the minimal promoter at -157/+135. DNase I footprint analysis revealed two complexes located within the gene's core promoter region, and site-directed mutagenesis identified two regions that were critical to maintain basal expression. EMSA identified the presence of five Sp elements within the immediate promoter region that are capable of binding members of the Sp family of proteins. Among the five Sp elements, one element appears to not bind Sp1, Sp2, or Sp3 while influencing the interaction of Sp proteins with an adjacent Sp site. Overexpression of either Sp1 or Sp3 augments activity of the minimal promoter in Sp-deficient Drosophila SL2 cells. In summary, we report on the characterization of the rat FcRn minimal promoter, including the characterization of five Sp elements within this region that interact with members of the Sp family of transcriptional factors and drive promoter activity in intestinal cell lines.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume286
Issue number6 49-6
DOIs
StatePublished - Jun 2004

Fingerprint

Sp Transcription Factors
Fc Receptors
Genetic Promoter Regions
Deoxyribonuclease I
Luciferases
Cell Line
Genes
DNA Footprinting
Clone Cells
Transcriptional Regulatory Elements
Caco-2 Cells
Site-Directed Mutagenesis
Drosophila
Transfection
Proteins

Keywords

  • Development
  • Immunoglobulin
  • Ontogeny
  • Passive immunity

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Characterization of the rat intestinal Fc receptor (FcRn) promoter : Transcriptional regulation of FcRn gene by the Sp family of transcription factors. / Jiang, Lingling; Wang, Jiafang; Solorzano-Vargas, R. Sergio; Tsai, Hugh V.; Gutierrez, Edgar M.; Ontiveros, Luis O.; Kiela, Pawel R; Wu, S. Vincent; Martín, Martín G.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 286, No. 6 49-6, 06.2004.

Research output: Contribution to journalArticle

Jiang, Lingling ; Wang, Jiafang ; Solorzano-Vargas, R. Sergio ; Tsai, Hugh V. ; Gutierrez, Edgar M. ; Ontiveros, Luis O. ; Kiela, Pawel R ; Wu, S. Vincent ; Martín, Martín G. / Characterization of the rat intestinal Fc receptor (FcRn) promoter : Transcriptional regulation of FcRn gene by the Sp family of transcription factors. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2004 ; Vol. 286, No. 6 49-6.
@article{bad5f1783aaf4692ad02a2b68331a7c7,
title = "Characterization of the rat intestinal Fc receptor (FcRn) promoter: Transcriptional regulation of FcRn gene by the Sp family of transcription factors",
abstract = "The regulatory elements that control the transcriptional regulation of the intestinal Fc receptor (FcRn) have not been elucidated. The objective of this study was to characterize the core promoter region of the rat FcRn gene. Chimeric clones that contained various regions of the promoter located upstream of the luciferase reporter were transiently transfected into either IEC-6 or Caco-2 cell lines and nuclear extracts were used to perform DNase I footprint and DNA binding assays (EMSA). Transfection of chimeric upstream nested deletions-luciferase reporter clones into either of these cell lines supported robust reporter activity and identified the location of the minimal promoter at -157/+135. DNase I footprint analysis revealed two complexes located within the gene's core promoter region, and site-directed mutagenesis identified two regions that were critical to maintain basal expression. EMSA identified the presence of five Sp elements within the immediate promoter region that are capable of binding members of the Sp family of proteins. Among the five Sp elements, one element appears to not bind Sp1, Sp2, or Sp3 while influencing the interaction of Sp proteins with an adjacent Sp site. Overexpression of either Sp1 or Sp3 augments activity of the minimal promoter in Sp-deficient Drosophila SL2 cells. In summary, we report on the characterization of the rat FcRn minimal promoter, including the characterization of five Sp elements within this region that interact with members of the Sp family of transcriptional factors and drive promoter activity in intestinal cell lines.",
keywords = "Development, Immunoglobulin, Ontogeny, Passive immunity",
author = "Lingling Jiang and Jiafang Wang and Solorzano-Vargas, {R. Sergio} and Tsai, {Hugh V.} and Gutierrez, {Edgar M.} and Ontiveros, {Luis O.} and Kiela, {Pawel R} and Wu, {S. Vincent} and Mart{\'i}n, {Mart{\'i}n G.}",
year = "2004",
month = "6",
doi = "10.1152/ajpgi.00131.2003",
language = "English (US)",
volume = "286",
journal = "American Journal of Physiology",
issn = "0363-6143",
publisher = "American Physiological Society",
number = "6 49-6",

}

TY - JOUR

T1 - Characterization of the rat intestinal Fc receptor (FcRn) promoter

T2 - Transcriptional regulation of FcRn gene by the Sp family of transcription factors

AU - Jiang, Lingling

AU - Wang, Jiafang

AU - Solorzano-Vargas, R. Sergio

AU - Tsai, Hugh V.

AU - Gutierrez, Edgar M.

AU - Ontiveros, Luis O.

AU - Kiela, Pawel R

AU - Wu, S. Vincent

AU - Martín, Martín G.

PY - 2004/6

Y1 - 2004/6

N2 - The regulatory elements that control the transcriptional regulation of the intestinal Fc receptor (FcRn) have not been elucidated. The objective of this study was to characterize the core promoter region of the rat FcRn gene. Chimeric clones that contained various regions of the promoter located upstream of the luciferase reporter were transiently transfected into either IEC-6 or Caco-2 cell lines and nuclear extracts were used to perform DNase I footprint and DNA binding assays (EMSA). Transfection of chimeric upstream nested deletions-luciferase reporter clones into either of these cell lines supported robust reporter activity and identified the location of the minimal promoter at -157/+135. DNase I footprint analysis revealed two complexes located within the gene's core promoter region, and site-directed mutagenesis identified two regions that were critical to maintain basal expression. EMSA identified the presence of five Sp elements within the immediate promoter region that are capable of binding members of the Sp family of proteins. Among the five Sp elements, one element appears to not bind Sp1, Sp2, or Sp3 while influencing the interaction of Sp proteins with an adjacent Sp site. Overexpression of either Sp1 or Sp3 augments activity of the minimal promoter in Sp-deficient Drosophila SL2 cells. In summary, we report on the characterization of the rat FcRn minimal promoter, including the characterization of five Sp elements within this region that interact with members of the Sp family of transcriptional factors and drive promoter activity in intestinal cell lines.

AB - The regulatory elements that control the transcriptional regulation of the intestinal Fc receptor (FcRn) have not been elucidated. The objective of this study was to characterize the core promoter region of the rat FcRn gene. Chimeric clones that contained various regions of the promoter located upstream of the luciferase reporter were transiently transfected into either IEC-6 or Caco-2 cell lines and nuclear extracts were used to perform DNase I footprint and DNA binding assays (EMSA). Transfection of chimeric upstream nested deletions-luciferase reporter clones into either of these cell lines supported robust reporter activity and identified the location of the minimal promoter at -157/+135. DNase I footprint analysis revealed two complexes located within the gene's core promoter region, and site-directed mutagenesis identified two regions that were critical to maintain basal expression. EMSA identified the presence of five Sp elements within the immediate promoter region that are capable of binding members of the Sp family of proteins. Among the five Sp elements, one element appears to not bind Sp1, Sp2, or Sp3 while influencing the interaction of Sp proteins with an adjacent Sp site. Overexpression of either Sp1 or Sp3 augments activity of the minimal promoter in Sp-deficient Drosophila SL2 cells. In summary, we report on the characterization of the rat FcRn minimal promoter, including the characterization of five Sp elements within this region that interact with members of the Sp family of transcriptional factors and drive promoter activity in intestinal cell lines.

KW - Development

KW - Immunoglobulin

KW - Ontogeny

KW - Passive immunity

UR - http://www.scopus.com/inward/record.url?scp=2642542441&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2642542441&partnerID=8YFLogxK

U2 - 10.1152/ajpgi.00131.2003

DO - 10.1152/ajpgi.00131.2003

M3 - Article

C2 - 15132949

AN - SCOPUS:2642542441

VL - 286

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 0363-6143

IS - 6 49-6

ER -