Characterization of the subset of immature thymocytes which can undergo rapid in vitro differentiation

Janko Nikolich-Zugich, M. W. Moore, M. J. Bevan

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Recently, we reported that thymocytes expressing the CD8 molecule on their surface can give rise to CD4+CD8+ double-positive and CD4+ single-positive progeny following intrathymic transfer into an irradiated host mouse. Thymocytes expressing a high density of CD8, referred to as CD8(hi), and those expressing a low density of the molecule, CD8(lo), were both able to differentiate in vivo. In this study we examined the ability of these CD8+ thymocyte populations and of CD4-CD8- double-negative thymocytes to change their phenotype during brief in vitro culture. CD8+ thymocytes were prepared by anti-CD4 plus complement lysis followed by positive selection of the survivors on anti-CD8-coated plates. After 16 h of culture, >60% of CD8+ thymocytes became double-positive. Both CD8(hi) and CD8(lo) cells were able to show this in vitro change: about 30% of the former and about 80% of the latter became double-positive. In contrast to this, double-negative thymocytes which had been depleted of cells expressing low densities of CD8 did not show such a phenotypic conversion in vitro. Further panning experiments suggested that all of the CD8+ thymocytes actually express a low surface density of the CD4 molecule which is undetectable in our cytofluorometric assays.

Original languageEnglish (US)
Pages (from-to)649-653
Number of pages5
JournalEuropean Journal of Immunology
Volume19
Issue number4
StatePublished - 1989
Externally publishedYes

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Thymocytes
CD4 Antigens
In Vitro Techniques
Phenotype
Population

ASJC Scopus subject areas

  • Immunology

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Characterization of the subset of immature thymocytes which can undergo rapid in vitro differentiation. / Nikolich-Zugich, Janko; Moore, M. W.; Bevan, M. J.

In: European Journal of Immunology, Vol. 19, No. 4, 1989, p. 649-653.

Research output: Contribution to journalArticle

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