Characterization of three novel sine families with unusual features in Helicoverpa armigera

Jianjun Wang, Aina Wang, Zhaojun Han, Zan Zhang, Fei Li, Xianchun Li

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Although more than 120 families of short interspersed nuclear elements (SINEs) have been isolated from the eukaryotic genomes, little is known about SINEs in insects. Here, we characterize three novel SINEs from the cotton bollworm, Helicoverpa armigera. Two of them, HaSE1 and HaSE2, share similar 5′ -structure including a tRNA-related region immediately followed by conserved central domain. The 3′ -tail of HaSE1 is significantly similar to that of one LINE retrotransposon element, HaRTE1.1, in H. armigera genome. The 3′ -region of HaSE2 showed high identity with one mariner-like element in H. armigera. The third family, termed HaSE3, is a 5S rRNA-derived SINE and shares both body part and 3′-tail with HaSE1, thus may represent the first example of a chimera generated by recombination between 5S rRNA and tRNA-derived SINE in insect species. Further database searches revealed the presence of these SINEs in several other related insect species, but not in the silkworm, Bombyx mori, indicating a relatively narrow distribution of these SINEs in Lepidopterans. Apart from above, we found a copy of HaSE2 in the GenBank EST entry for the cotton aphid, Aphis gossypii, suggesting the occurrence of horizontal transfer.

Original languageEnglish (US)
Article numbere31355
JournalPLoS One
Volume7
Issue number2
DOIs
StatePublished - Feb 3 2012

Fingerprint

Helicoverpa armigera
Transfer RNA
Cotton
Insects
Bombyx
Genes
Retroelements
Aphis gossypii
Tail
Expressed Sequence Tags
Genome
insects
Aphids
tail
Nucleic Acid Databases
ribosomal RNA
Human Body
Genetic Recombination
genome
chimerism

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Characterization of three novel sine families with unusual features in Helicoverpa armigera. / Wang, Jianjun; Wang, Aina; Han, Zhaojun; Zhang, Zan; Li, Fei; Li, Xianchun.

In: PLoS One, Vol. 7, No. 2, e31355, 03.02.2012.

Research output: Contribution to journalArticle

Wang, Jianjun ; Wang, Aina ; Han, Zhaojun ; Zhang, Zan ; Li, Fei ; Li, Xianchun. / Characterization of three novel sine families with unusual features in Helicoverpa armigera. In: PLoS One. 2012 ; Vol. 7, No. 2.
@article{6137334c56b44e8f810c69a54cd44283,
title = "Characterization of three novel sine families with unusual features in Helicoverpa armigera",
abstract = "Although more than 120 families of short interspersed nuclear elements (SINEs) have been isolated from the eukaryotic genomes, little is known about SINEs in insects. Here, we characterize three novel SINEs from the cotton bollworm, Helicoverpa armigera. Two of them, HaSE1 and HaSE2, share similar 5′ -structure including a tRNA-related region immediately followed by conserved central domain. The 3′ -tail of HaSE1 is significantly similar to that of one LINE retrotransposon element, HaRTE1.1, in H. armigera genome. The 3′ -region of HaSE2 showed high identity with one mariner-like element in H. armigera. The third family, termed HaSE3, is a 5S rRNA-derived SINE and shares both body part and 3′-tail with HaSE1, thus may represent the first example of a chimera generated by recombination between 5S rRNA and tRNA-derived SINE in insect species. Further database searches revealed the presence of these SINEs in several other related insect species, but not in the silkworm, Bombyx mori, indicating a relatively narrow distribution of these SINEs in Lepidopterans. Apart from above, we found a copy of HaSE2 in the GenBank EST entry for the cotton aphid, Aphis gossypii, suggesting the occurrence of horizontal transfer.",
author = "Jianjun Wang and Aina Wang and Zhaojun Han and Zan Zhang and Fei Li and Xianchun Li",
year = "2012",
month = "2",
day = "3",
doi = "10.1371/journal.pone.0031355",
language = "English (US)",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "2",

}

TY - JOUR

T1 - Characterization of three novel sine families with unusual features in Helicoverpa armigera

AU - Wang, Jianjun

AU - Wang, Aina

AU - Han, Zhaojun

AU - Zhang, Zan

AU - Li, Fei

AU - Li, Xianchun

PY - 2012/2/3

Y1 - 2012/2/3

N2 - Although more than 120 families of short interspersed nuclear elements (SINEs) have been isolated from the eukaryotic genomes, little is known about SINEs in insects. Here, we characterize three novel SINEs from the cotton bollworm, Helicoverpa armigera. Two of them, HaSE1 and HaSE2, share similar 5′ -structure including a tRNA-related region immediately followed by conserved central domain. The 3′ -tail of HaSE1 is significantly similar to that of one LINE retrotransposon element, HaRTE1.1, in H. armigera genome. The 3′ -region of HaSE2 showed high identity with one mariner-like element in H. armigera. The third family, termed HaSE3, is a 5S rRNA-derived SINE and shares both body part and 3′-tail with HaSE1, thus may represent the first example of a chimera generated by recombination between 5S rRNA and tRNA-derived SINE in insect species. Further database searches revealed the presence of these SINEs in several other related insect species, but not in the silkworm, Bombyx mori, indicating a relatively narrow distribution of these SINEs in Lepidopterans. Apart from above, we found a copy of HaSE2 in the GenBank EST entry for the cotton aphid, Aphis gossypii, suggesting the occurrence of horizontal transfer.

AB - Although more than 120 families of short interspersed nuclear elements (SINEs) have been isolated from the eukaryotic genomes, little is known about SINEs in insects. Here, we characterize three novel SINEs from the cotton bollworm, Helicoverpa armigera. Two of them, HaSE1 and HaSE2, share similar 5′ -structure including a tRNA-related region immediately followed by conserved central domain. The 3′ -tail of HaSE1 is significantly similar to that of one LINE retrotransposon element, HaRTE1.1, in H. armigera genome. The 3′ -region of HaSE2 showed high identity with one mariner-like element in H. armigera. The third family, termed HaSE3, is a 5S rRNA-derived SINE and shares both body part and 3′-tail with HaSE1, thus may represent the first example of a chimera generated by recombination between 5S rRNA and tRNA-derived SINE in insect species. Further database searches revealed the presence of these SINEs in several other related insect species, but not in the silkworm, Bombyx mori, indicating a relatively narrow distribution of these SINEs in Lepidopterans. Apart from above, we found a copy of HaSE2 in the GenBank EST entry for the cotton aphid, Aphis gossypii, suggesting the occurrence of horizontal transfer.

UR - http://www.scopus.com/inward/record.url?scp=84863025405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863025405&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0031355

DO - 10.1371/journal.pone.0031355

M3 - Article

C2 - 22319625

AN - SCOPUS:84863025405

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 2

M1 - e31355

ER -