Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications

Timothy S. Frost; Linan Jiang; Yitshak Zohar

doi:10.1109/NANOMED.2018.8641558

Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications

Timothy S. Frost, Linan Jiang, Yitshak Zohar

Aerospace and Mechanical Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.

Original language	English (US)
Title of host publication	Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018
Publisher	IEEE Computer Society
Pages	27-30
Number of pages	4
ISBN (Electronic)	9781538675786
DOIs	https://doi.org/10.1109/NANOMED.2018.8641558
State	Published - Feb 13 2019
Event	12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018 - Waikiki Beach, United States Duration: Dec 2 2018 → Dec 5 2018

Publication series

Name	IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
Volume	2018-December
ISSN (Print)	2159-6964
ISSN (Electronic)	2159-6972

Conference

Conference	12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018
Country	United States
City	Waikiki Beach
Period	12/2/18 → 12/5/18

Keywords

A549 cell line
Convection-diffusion flow
Pharmacokinetics
Protein transport

ASJC Scopus subject areas

Molecular Medicine
Biomedical Engineering
Microbiology (medical)

Access to Document

10.1109/NANOMED.2018.8641558

Fingerprint Dive into the research topics of 'Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications'. Together they form a unique fingerprint.

Cite this

Frost, T. S., Jiang, L., & Zohar, Y. (2019). Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications. In Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018 (pp. 27-30). [8641558] (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED; Vol. 2018-December). IEEE Computer Society. https://doi.org/10.1109/NANOMED.2018.8641558

Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications. / Frost, Timothy S.; Jiang, Linan; Zohar, Yitshak.

Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018. IEEE Computer Society, 2019. p. 27-30 8641558 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED; Vol. 2018-December).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Frost, TS, Jiang, L & Zohar, Y 2019, Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications. in Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018., 8641558, IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED, vol. 2018-December, IEEE Computer Society, pp. 27-30, 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018, Waikiki Beach, United States, 12/2/18. https://doi.org/10.1109/NANOMED.2018.8641558

Frost TS, Jiang L, Zohar Y. Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications. In Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018. IEEE Computer Society. 2019. p. 27-30. 8641558. (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED). https://doi.org/10.1109/NANOMED.2018.8641558

Frost, Timothy S. ; Jiang, Linan ; Zohar, Yitshak. / Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications. Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018. IEEE Computer Society, 2019. pp. 27-30 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED).

@inproceedings{05228c1c29c445f3aeacac6ea6f743ea,

title = "Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications",

abstract = "Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.",

keywords = "A549 cell line, Convection-diffusion flow, Pharmacokinetics, Protein transport",

author = "Frost, {Timothy S.} and Linan Jiang and Yitshak Zohar",

year = "2019",

month = feb,

day = "13",

doi = "10.1109/NANOMED.2018.8641558",

language = "English (US)",

series = "IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED",

publisher = "IEEE Computer Society",

pages = "27--30",

booktitle = "Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018",

note = "12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018 ; Conference date: 02-12-2018 Through 05-12-2018",

}

TY - GEN

T1 - Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications

AU - Frost, Timothy S.

AU - Jiang, Linan

AU - Zohar, Yitshak

PY - 2019/2/13

Y1 - 2019/2/13

N2 - Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.

AB - Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.

KW - A549 cell line

KW - Convection-diffusion flow

KW - Pharmacokinetics

KW - Protein transport

UR - http://www.scopus.com/inward/record.url?scp=85063040141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063040141&partnerID=8YFLogxK

U2 - 10.1109/NANOMED.2018.8641558

DO - 10.1109/NANOMED.2018.8641558

M3 - Conference contribution

AN - SCOPUS:85063040141

T3 - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED

SP - 27

EP - 30

BT - Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018

PB - IEEE Computer Society

T2 - 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018

Y2 - 2 December 2018 through 5 December 2018

ER -