Abstract
Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.
| Original language | English (US) |
|---|---|
| Title of host publication | Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018 |
| Publisher | IEEE Computer Society |
| Pages | 27-30 |
| Number of pages | 4 |
| ISBN (Electronic) | 9781538675786 |
| DOIs | |
| State | Published - Feb 13 2019 |
| Event | 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018 - Waikiki Beach, United States Duration: Dec 2 2018 → Dec 5 2018 |
Publication series
| Name | IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED |
|---|---|
| Volume | 2018-December |
| ISSN (Print) | 2159-6964 |
| ISSN (Electronic) | 2159-6972 |
Conference
| Conference | 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018 |
|---|---|
| Country | United States |
| City | Waikiki Beach |
| Period | 12/2/18 → 12/5/18 |
Fingerprint
Keywords
- A549 cell line
- Convection-diffusion flow
- Pharmacokinetics
- Protein transport
ASJC Scopus subject areas
- Molecular Medicine
- Biomedical Engineering
- Microbiology (medical)
Cite this
Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications. / Frost, Timothy S.; Jiang, Linan; Zohar, Yitshak.
Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018. IEEE Computer Society, 2019. p. 27-30 8641558 (IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED; Vol. 2018-December).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications
AU - Frost, Timothy S.
AU - Jiang, Linan
AU - Zohar, Yitshak
PY - 2019/2/13
Y1 - 2019/2/13
N2 - Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.
AB - Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate of larger molecules with little effect on the paracellular transport of smaller molecules. The results indicate that the microfluidic system is a good model for pharmacokinetic applications.
KW - A549 cell line
KW - Convection-diffusion flow
KW - Pharmacokinetics
KW - Protein transport
UR - http://www.scopus.com/inward/record.url?scp=85063040141&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85063040141&partnerID=8YFLogxK
U2 - 10.1109/NANOMED.2018.8641558
DO - 10.1109/NANOMED.2018.8641558
M3 - Conference contribution
AN - SCOPUS:85063040141
T3 - IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
SP - 27
EP - 30
BT - Proceedings of the 12th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2018
PB - IEEE Computer Society
ER -