Chemical and bioactive natural products from microthyriaceae sp., an endophytic fungus from a tropical grass

C. Almeida, H. Ortega, S. Higginbotham, C. Spadafora, A. E. Arnold, P. D. Coley, T. A. Kursar, W. H. Gerwick, L. Cubilla-Rios

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

In screening for natural products with antiparasitic activity, an endophytic fungus, strain F2611, isolated from above-ground tissue of the tropical grass Paspalum conjugatum (Poaceae) in Panama, was chosen for bioactive principle elucidation. Cultivation on malt extract agar (MEA) followed by bioassay-guided chromatographic fractionation of the extract led to the isolation of the new polyketide integrasone B (1) and two known mycotoxins, sterigmatocystin (2) and secosterigmatocystin (3). Sterigmatocystin (2) was found to be the main antiparasitic compound in the fermentation extract of this fungus, possessing potent and selective antiparasitic activity against Trypanosoma cruzi, the cause of Chagas disease, with an IC50 value of 0·13 μmol l-1. Compounds 2 and 3 showed high cytotoxicity against Vero cells (IC50 of 0·06 and 0·97 μmol l-1, respectively). The new natural product integrasone B (1), which was co-purified from the active fractions, constitutes the second report of a natural product possessing an epoxyquinone with a lactone ring and exhibited no significant biological activity. Strain F2611 represents a previously undescribed taxon within the Microthyriaceae (Dothideomycetes, Ascomycota).

Original languageEnglish (US)
Pages (from-to)58-64
Number of pages7
JournalLetters in Applied Microbiology
Volume59
Issue number1
DOIs
StatePublished - Jul 2014

Keywords

  • Antiparasitic activity
  • Endophytic fungi
  • Integrasone B
  • Sterigmatocystin

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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    Almeida, C., Ortega, H., Higginbotham, S., Spadafora, C., Arnold, A. E., Coley, P. D., Kursar, T. A., Gerwick, W. H., & Cubilla-Rios, L. (2014). Chemical and bioactive natural products from microthyriaceae sp., an endophytic fungus from a tropical grass. Letters in Applied Microbiology, 59(1), 58-64. https://doi.org/10.1111/lam.12245