Chemical bromination of phenol red by hydrogen peroxide is possible in the absence of haloperoxidases

María C. Terrón, Frank J.M. Verhagen, Maurice C.R. Franssen, Jim A. Field

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The dye phenol red (phenolsulphonphthalein) was chemically brominated to bromophenol blue (3',3',5',5'-tetrabromophenolsulphonphthalein) directly by hydrogen peroxide (20 mM or higher) under acidic pH (0.5-4.5) and moderate temperature (30°C). Since this bromination reaction takes place in the absence of any chemical or biological catalyst, the results represent an important warning concerning a commonly used assay for detecting or screening haloperoxidases. Bromophenol red (5',5'-dibromophenolsulphonphthalein) and another unidentified compound were observed as temporary intermediates. Incubation of the reaction mixture with KCl instead of NaBr did not yield any chlorinated products under the same conditions.

Original languageEnglish (US)
Pages (from-to)1445-1452
Number of pages8
JournalChemosphere
Volume36
Issue number6
DOIs
StatePublished - Mar 1 1998
Externally publishedYes

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Chemistry(all)
  • Pollution
  • Health, Toxicology and Mutagenesis

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