Analyses of total 3-hydroxyproline and 4-hydroxyproline in tissue samples can be used to estimate the quantity of two kinds of collagen: that of basement-membrane and that of the several interstitial Types. Applied to an experimental model for obstructive nephropathy in the rabbit, such data show that the large increase in collagen in the renal cortex (at the 32nd day following unilateral ureteral ligation) is primarily of interstitial type. However there is also an appreciable increase in basement membrane collagen. Both findings are in agreement with the qualitative impression from electronmicrographs. The chemical method offers an approach to quantitation of these two kinds of collagen (basement membrane and interestitial) in normal and diseased tissues.
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Molecular Biology
- Clinical Biochemistry