Chemically defned generation of human cardiomyocytes

Paul W. Burridge, Elena Matsa, Praveen Shukla, Ziliang C. Lin, Jared M. Churko, Antje D. Ebert, Feng Lan, Sebastian Diecke, Bruno Huber, Nicholas M. Mordwinkin, Jordan R. Plews, Oscar J. Abilez, Bianxiao Cui, Joseph D. Gold, Joseph C. Wu

Research output: Contribution to journalArticle

481 Citations (Scopus)

Abstract

Existing methods for human induced pluripotent stem cell (hiPsc) cardiac differentiation are effcient but require complex, undefned medium constituents that hinder further elucidation of the molecular mechanisms of cardiomyogenesis. using hiPscs derived under chemically defned conditions on synthetic matrices, we systematically developed an optimized cardiac differentiation strategy, using a chemically defned medium consisting of just three components: the basal medium rPmi 1640, l-ascorbic acid 2-phosphate and rice-derived recombinant human albumin. Along with small molecule-based induction of differentiation, this protocol produced contractile sheets of up to 95% tnnt2+ cardiomyocytes at a yield of up to 100 cardiomyocytes for every input pluripotent cell and was effective in 11 hiPsc lines tested. this chemically defned platform for cardiac specifcation of hiPscs will allow the elucidation of cardiomyocyte macromolecular and metabolic requirements and will provide a minimal system for the study of maturation and subtype specifcation.

Original languageEnglish (US)
Pages (from-to)855-860
Number of pages6
JournalNature Methods
Volume11
Issue number8
DOIs
StatePublished - Aug 2014
Externally publishedYes

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Stem cells
Cardiac Myocytes
Induced Pluripotent Stem Cells
Albumins
Molecules
Cell Differentiation
Cell Line
ascorbate-2-phosphate

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Burridge, P. W., Matsa, E., Shukla, P., Lin, Z. C., Churko, J. M., Ebert, A. D., ... Wu, J. C. (2014). Chemically defned generation of human cardiomyocytes. Nature Methods, 11(8), 855-860. https://doi.org/10.1038/nMeth.2999

Chemically defned generation of human cardiomyocytes. / Burridge, Paul W.; Matsa, Elena; Shukla, Praveen; Lin, Ziliang C.; Churko, Jared M.; Ebert, Antje D.; Lan, Feng; Diecke, Sebastian; Huber, Bruno; Mordwinkin, Nicholas M.; Plews, Jordan R.; Abilez, Oscar J.; Cui, Bianxiao; Gold, Joseph D.; Wu, Joseph C.

In: Nature Methods, Vol. 11, No. 8, 08.2014, p. 855-860.

Research output: Contribution to journalArticle

Burridge, PW, Matsa, E, Shukla, P, Lin, ZC, Churko, JM, Ebert, AD, Lan, F, Diecke, S, Huber, B, Mordwinkin, NM, Plews, JR, Abilez, OJ, Cui, B, Gold, JD & Wu, JC 2014, 'Chemically defned generation of human cardiomyocytes', Nature Methods, vol. 11, no. 8, pp. 855-860. https://doi.org/10.1038/nMeth.2999
Burridge PW, Matsa E, Shukla P, Lin ZC, Churko JM, Ebert AD et al. Chemically defned generation of human cardiomyocytes. Nature Methods. 2014 Aug;11(8):855-860. https://doi.org/10.1038/nMeth.2999
Burridge, Paul W. ; Matsa, Elena ; Shukla, Praveen ; Lin, Ziliang C. ; Churko, Jared M. ; Ebert, Antje D. ; Lan, Feng ; Diecke, Sebastian ; Huber, Bruno ; Mordwinkin, Nicholas M. ; Plews, Jordan R. ; Abilez, Oscar J. ; Cui, Bianxiao ; Gold, Joseph D. ; Wu, Joseph C. / Chemically defned generation of human cardiomyocytes. In: Nature Methods. 2014 ; Vol. 11, No. 8. pp. 855-860.
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