Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography

Paul B Myrdal, B. Steven Angersbach, Kelly Karlage, Philip J. Kuehl

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An isocratic, reversed-phase HPLC assay has been developed for the separation of the enantiomers of four lipoxygenase metabolites, without the need for a derivatization step. Separation of the enantiomers was studied on a polysaccharide type chiral stationary phase column. Upon determination of suitable mobile phase composition, the assay was evaluated at various temperatures. In all cases the R enantiomer eluted before the S enantiomer. The best separations were observed at 0 °C.

Original languageEnglish (US)
Pages (from-to)315-319
Number of pages5
JournalJournal of Chromatography A
Volume1132
Issue number1-2
DOIs
StatePublished - Nov 3 2006

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Lipoxygenase
Enantiomers
High performance liquid chromatography
Metabolites
Polysaccharides
High Pressure Liquid Chromatography
Temperature
Assays
Phase composition

Keywords

  • Arachidonic acid metabolism
  • Chiral separation
  • Chiralcel OR-RH
  • HETEs
  • Lipoxygenase

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography. / Myrdal, Paul B; Angersbach, B. Steven; Karlage, Kelly; Kuehl, Philip J.

In: Journal of Chromatography A, Vol. 1132, No. 1-2, 03.11.2006, p. 315-319.

Research output: Contribution to journalArticle

Myrdal, Paul B ; Angersbach, B. Steven ; Karlage, Kelly ; Kuehl, Philip J. / Chiral separation of lipoxygenase metabolites utilizing high-performance liquid chromatography. In: Journal of Chromatography A. 2006 ; Vol. 1132, No. 1-2. pp. 315-319.
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