Chlamydia trachomatis and Pap testing from a single, fluid-based sample: A multicenter study

S. L. Inhorn, P. J. Wand, T. C. Wright, K. D. Hatch, J. Hallum, B. B. Lentrichia

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

OBJECTIVE: To determine the potential for both Pap testing and the Chlamydia direct fluorescence assay (DFA) from a single sample using the fluid-based ThinPrep® Pap Test™ method (Cytyc Corporation, Boxborough, Massachusetts). STUDY DESIGN: Conventional DFA was compared to ThinPrep® DFA in a direct-to-vial, double-blinded, multicenter protocol. Cervical scrapings were collected for the ThinPrep® Pap Test, and then a second swab was used to collect an endocervical sample for a conventional DFA test. The DFA slide prepared from the ThinPrep® Test and the conventional DFA sample prepared from the endocervical swab were evaluated independently. Discrepant cases were adjudicated by testing residual specimens using a Chlamydia direct DNA method. RESULTS: Combining 636 adequate cases (94% of the total collected), 582 (91.5%) were negative on both slides, 43 (6.8%) positive by both and 11 (1.7%) discrepant. The prevalence of Chlamydia was 7.9% based on the conventional DFA method (range, 4.3-10.9%). McNemar's two-tailed test indicated the results not to be statistically different (P>.05). Adjudication favored ThinPrep® 45% of the time and conventional 55%. Specimen adequacy favored ThinPrep with high statistical significance (McNemar's test, P>.01). CONCLUSION: A second slide prepared from the same vial of cells as that used for the ThinPrep® Pap Test can be used for Chlamydia testing by DFA. Fluid-based collection could allow multiple tests from a single sample.

Original languageEnglish (US)
Pages (from-to)237-242
Number of pages6
JournalJournal of Reproductive Medicine for the Obstetrician and Gynecologist
Volume46
Issue number3
StatePublished - Apr 25 2001
Externally publishedYes

Keywords

  • Cervical smears
  • Cervix diseases
  • Chlamydia trachomatis
  • Papanicolaou smear
  • ThinPrep® Pap Test™

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

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